Search International and National Patent Collections

1. (WO2010114686) MODULATION OF ABCG2-MEDIATED URATE TRANSPORT TO TREAT HYPERURICEMIA AND GOUT

Pub. No.:    WO/2010/114686    International Application No.:    PCT/US2010/026932
Publication Date: Fri Oct 08 01:59:59 CEST 2010 International Filing Date: Fri Mar 12 00:59:59 CET 2010
IPC: G01N 33/50
G01N 33/15
C12Q 1/68
Applicants: JOHNS HOPKINS UNIVERSITY
KOTTGEN, Michael
CORESH, Josef
GUGGINO, William, B.
KOTTGEN, Anna
WOODWARD, Owen, M.
Inventors: KOTTGEN, Michael
CORESH, Josef
GUGGINO, William, B.
KOTTGEN, Anna
WOODWARD, Owen, M.
Title: MODULATION OF ABCG2-MEDIATED URATE TRANSPORT TO TREAT HYPERURICEMIA AND GOUT
Abstract:
Genome-wide association studies (GWAS) was recently used to identify SNPs in a genomic region on chromosome 4 that associate with serum urate levels and gout. The present disclosure shows that human ATP-binding cassette, subfamily G, 2 (ABCG2), encoded by the ABCG2 gene contained in this region, is a hitherto unknown urate efflux transporter. The present disclosure further shows that native ABCG2 is located in the brush border membrane of kidney proximal tubule cells, where it mediates renal urate secretion. Introduction of the mutation Q 141 K encoded by the common SNP rs2231142 by site-directed mutagenesis resulted in reduced urate transport rates compared to wild-type ABCG2. Data from a population-based study of 14,783 individuals support rs2231142 as the causal variant in the region and show highly significant associations with urate levels and gout.