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1. (WO2010114532) SOLUBILIZATION AND PURIFICATION OF A TARGET PROTEIN FUSED TO A MUTANT MALTOSE-BINDING PROTEIN

Pub. No.:    WO/2010/114532    International Application No.:    PCT/US2009/039111
Publication Date: Fri Oct 08 01:59:59 CEST 2010 International Filing Date: Thu Apr 02 01:59:59 CEST 2009
IPC: C07K 14/245
Applicants: NEW ENGLAND BIOLABS, INC.
RIGGS, Paul
WALKER, Iris
COLUSSI, Paul, A.
GANATRA, Mehul
TARON, Christopher, H.
Inventors: RIGGS, Paul
WALKER, Iris
COLUSSI, Paul, A.
GANATRA, Mehul
TARON, Christopher, H.
Title: SOLUBILIZATION AND PURIFICATION OF A TARGET PROTEIN FUSED TO A MUTANT MALTOSE-BINDING PROTEIN
Abstract:
Methods and compositions are provided that relate to a composition that includes a modified maltose-binding protein (MBP) which when fused to a protein results in an increase in binding affinity for maltodextrin compared with the wild type MBP fused to the protein, the modified MBP maintaining enhanced solubility, The modification includes a mutation selected from the group consisting of : F68L, I318V, Q326R, V344M, and T372TTTITITTTLGIEGR387 or consist of two or more mutations selected from the group consisting of : F68L, S146T, A313V, I318V, I318A, Q326R, V344M and T372TTTITITTTLGIEGR387 mutants.