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1. (WO2010113832) CORYNEFORM BACTERIUM TRANSFORMANT, AND PROCESS FOR PRODUCING ISOBUTANOL USING SAME
Latest bibliographic data on file with the International Bureau   

Pub. No.: WO/2010/113832 International Application No.: PCT/JP2010/055504
Publication Date: 07.10.2010 International Filing Date: 29.03.2010
IPC:
C12N 1/21 (2006.01) ,C12N 15/09 (2006.01) ,C12P 7/16 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1
Micro-organisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving micro-organisms or compositions thereof; Processes of preparing or isolating a composition containing a micro-organism; Culture media therefor
20
Bacteria; Culture media therefor
21
modified by introduction of foreign genetic material
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
P
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
7
Preparation of oxygen-containing organic compounds
02
containing a hydroxy group
04
acyclic
16
Butanols
Applicants: YUKAWA, Hideaki[JP/JP]; JP (UsOnly)
INUI, Masayuki[JP/JP]; JP (UsOnly)
RESEARCH INSTITUTE OF INNOVATIVE TECHNOLOGY FOR THE EARTH[JP/JP]; 9-2, Kizugawadai, Kizugawa-shi, Kyoto 6190292, JP (AllExceptUS)
Inventors: YUKAWA, Hideaki; JP
INUI, Masayuki; JP
Agent: IWATANI, Ryo; KEIHAN Dojima Bldg. 3F, 1-31, Dojima 2-chome, Kita-ku, Osaka-shi, Osaka 5300003, JP
Priority Data:
2009-08366830.03.2009JP
Title (EN) CORYNEFORM BACTERIUM TRANSFORMANT, AND PROCESS FOR PRODUCING ISOBUTANOL USING SAME
(FR) TRANSFORMANT DE BACTÉRIE CORYNÉFORME, ET PROCÉDÉ DE PRODUCTION D'ISOBUTANOL L'UTILISANT
(JA) コリネ型細菌形質転換体及びそれを用いるイソブタノールの製造方法
Abstract:
(EN) A transformant capable of producing isobutanol, which is characterized by comprising Corynebacterium glutamicum having the genes (1) to (5) mentioned below, wherein at least one of the genes (1) to (5) is an endogenous gene of Corynebacterium glutamicum and at least one of the genes (1) to (5) is a foreign gene: (1) a gene encoding an enzyme having an acetohydroxy acid synthase activity; (2) a gene encoding an enzyme having an acetohydroxy acid isomeroreductase activity; (3) a gene encoding an enzyme having a dihydroxy acid dehydratase activity; (4) a gene encoding an enzyme having a 2-keto acid decarboxylase activity; and (5) a gene encoding an enzyme having an alcohol dehydrogenase activity.
(FR) L'invention porte sur un transformant capable de produire de l'isobutanol, qui est caractérisé en ce qu'il comprend Corynebacterium glutamicum ayant les gènes (1) à (5) mentionnés ci-après, au moins l'un des gènes (1) à (5) étant un gène endogène de Corynebacterium glutamicum et au moins l'un des gènes (1) à (5) étant un gène étranger : (1) un gène codant pour une enzyme ayant une activité acétohydroxy acide synthase ; (2) un gène codant pour une enzyme ayant une activité acétohydroxy acide isoméroréductase ; (3) un gène codant pour une enzyme ayant une activité dihydroxy acide déhydratase ; (4) un gène codant pour une enzyme ayant une activité 2-céto acide décarboxylase ; et (5) un gène codant pour une enzyme ayant une activité alcool déshydrogénase.
(JA)  下記の(1)~(5)の遺伝子を有するコリネバクテリウム グルタミカム(Corynebacterium glutamicum)であって、(1)~(5)の1以上がコリネバクテリウム グルタミカムの内在性の遺伝子であり、(1)~(5)の1以上が外来性の遺伝子であることを特徴とする、イソブタノール生産能を有する形質転換体。(1)アセトヒドロキシ酸 シンターゼ(acetohydroxy acid synthase)活性を有する酵素をコードする遺伝子 (2)アセトヒドロキシ酸 イソメロレダクターゼ(acetohydroxy acid isomeroreductase)活性を有する酵素をコードする遺伝子 (3)ジヒドロキシ酸 デヒドラターゼ(dihydroxy acid dehydratase)活性を有する酵素をコードする遺伝子 (4)2-ケト酸 デカルボキシラーゼ(2-keto acid decarboxylase)活性を有する酵素をコードする遺伝子 (5)アルコール デヒドロゲナーゼ(alcohol dehydrogenase)活性を有する酵素をコードする遺伝子
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PE, PG, PH, PL, PT, RO, RS, RU, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LR, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (EPO) (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG)
Publication Language: Japanese (JA)
Filing Language: Japanese (JA)