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1. WO2010097623 - TRANSGENIC PLANTS COMPRISING CONSTRUCTS ENCODING PHOSPHOENOLPYRUVATE CARBOXYKINASE AND/OR PYRUVATE ORTHO PHOS PHATE DIKINASE

Publication Number WO/2010/097623
Publication Date 02.09.2010
International Application No. PCT/GB2010/050321
International Filing Date 25.02.2010
Chapter 2 Demand Filed 25.11.2010
IPC
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
63
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79
Vectors or expression systems specially adapted for eukaryotic hosts
82
for plant cells
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
11
DNA or RNA fragments; Modified forms thereof
29
Genes encoding plant proteins, e.g. thaumatin
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9
Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
10
Transferases (2.)
12
transferring phosphorus containing groups, e.g. kinases (2.7)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9
Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
88
Lyases (4.)
A HUMAN NECESSITIES
24
TOBACCO; CIGARS; CIGARETTES; SMOKERS' REQUISITES
B
MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
15
Chemical features or treatment of tobacco; Tobacco substitutes
A HUMAN NECESSITIES
01
AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
H
NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
5
Flowering plants, i.e. angiosperms
A HUMAN NECESSITIES
01
AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
H
NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
5
Flowering plants, i.e. angiosperms
12
Leaves
C12N 15/82 (2006.01)
C12N 15/29 (2006.01)
C12N 9/12 (2006.01)
C12N 9/88 (2006.01)
A24B 15/00 (2006.01)
A01H 5/00 (2006.01)
CPC
A01H 5/12
A24B 15/10
A24B 15/245
C12N 15/8266
Applicants
  • ADVANCED TECHNOLOGIES (CAMBRIDGE) LIMITED [GB/GB]; Globe House 1 Water Street London WC2R 3LA, GB (AllExceptUS)
  • HIBBERD, Julian Michael [GB/GB]; GB (UsOnly)
  • TAYLOR, Lucy Elisabeth [GB/GB]; GB (UsOnly)
  • LEISS, Anna Elizabeth [GB/GB]; GB (UsOnly)
Inventors
  • HIBBERD, Julian Michael; GB
  • TAYLOR, Lucy Elisabeth; GB
  • LEISS, Anna Elizabeth; GB
Agents
  • HUTTER, Anton; Venner Shipley LLP 200 Aldersgate London EC1A 4HD, GB
Priority Data
0903346.527.02.2009GB
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) TRANSGENIC PLANTS COMPRISING CONSTRUCTS ENCODING PHOSPHOENOLPYRUVATE CARBOXYKINASE AND/OR PYRUVATE ORTHO PHOS PHATE DIKINASE
(FR) PLANTES TRANSGÉNIQUES COMPRENANT DES CONSTRUCTIONS CODANT POUR LA PHOSPHOÉNOLPYRUVATE CARBOXYKINASE ET/OU LA PYRUVATE ORTHOPHOSPHATE DIKINASE
Abstract
(EN)
The invention provides genetic constructs which comprise a senescence-specific promoter operably linked to at least one coding sequence, which encodes at least one polypeptide having phosphoenolpyruvate carboxykinase (PCK) activity and/or pyruvate orthophosphate dikinase (PPDK) activity.The constructs have the ability to cause, in transgenic plants, remobilisation of nitrogen during leaf senescence, such that nitrogen can be transported from the leaves to other regions of the plants. The invention provides plant cells and plants transformed with such constructs, methods of producing transgenic plants, and methods of increasing the rate of nitrogen remobilisation and growth rate in senescent plants. The invention also provides harvested plant leaves, such as tobacco leaves, that have been transformed with the genetic constructs, and to smoking articles comprising such harvested plant leaves.
(FR)
La présente invention concerne des constructions génétiques qui comprennent un promoteur spécifique de sénescence fonctionnellement lié à au moins une séquence codante, qui code pour au moins un polypeptide ayant une activité phosphoénolpyruvate carboxykinase (PCK) et/ou une activité pyruvate orthophosphate dikinase (PPDK). Les constructions ont la capacité de causer, dans des plantes transgéniques, la remobilisation de l'azote pendant la sénescence des feuilles, de sorte que l'azote puisse être transporté depuis les feuilles vers d'autres régions des plantes. L'invention concerne des cellules de plante et des plantes transformées avec de telles constructions, des procédés de production de plantes transgéniques, et des procédés d'augmentation du taux de remobilisation d'azote et du taux de croissance dans des plantes sénescentes. L'invention concerne en outre des feuilles de plante récoltées, telles que des feuilles de tabac, qui ont été transformées avec les constructions génétiques, et des articles pour fumeur comprenant de telles feuilles de plante récoltées.
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