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1. (WO2010084171) METHOD FOR TEMPORALLY CONTROLLING THE BIOLOGICAL ACTIVITY OF PROTEINS IN VERTEBRATES, AND APPLICATIONS THEREOF
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/2010/084171 International Application No.: PCT/EP2010/050723
Publication Date: 29.07.2010 International Filing Date: 22.01.2010
IPC:
C07K 14/72 (2006.01) ,C12N 9/00 (2006.01) ,C12N 15/90 (2006.01) ,C12N 9/90 (2006.01)
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
14
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435
from animals; from humans
705
Receptors; Cell surface antigens; Cell surface determinants
72
for hormones
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9
Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
87
Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
90
Stable introduction of foreign DNA into chromosome
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9
Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
90
Isomerases (5.)
Applicants:
CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE (CNRS) [FR/FR]; 3, rue Michel Ange F-75016 Paris, FR (AllExceptUS)
THE BOARD OF TRUSTEES OF THE UNIVERSITY OF ILLINOIS [US/US]; 352 Henry Administration Building MC350 506.S. Wright Street Urbana, Illinois 61801-3642, US (AllExceptUS)
UNIVERSITE DE STRASBOURG [FR/FR]; 4 rue Blaise Pascal F-67000 Strasbourg, FR (AllExceptUS)
METZGER, Daniel [FR/FR]; FR (UsOnly)
CHAMBON, Pierre [FR/FR]; FR (UsOnly)
ZHAO, Huimin [US/US]; US (UsOnly)
KATZENELLENBOGEN, John [US/US]; US (UsOnly)
Inventors:
METZGER, Daniel; FR
CHAMBON, Pierre; FR
ZHAO, Huimin; US
KATZENELLENBOGEN, John; US
Agent:
WARCOIN, Jacques; Cabinet Regimbeau 20, rue de Chazelles F-75847 Paris Cedex 17, FR
Priority Data:
61/146,44922.01.2009US
Title (EN) METHOD FOR TEMPORALLY CONTROLLING THE BIOLOGICAL ACTIVITY OF PROTEINS IN VERTEBRATES, AND APPLICATIONS THEREOF
(FR) PROCÉDÉ POUR CONTRÔLER TEMPORAIREMENT L'ACTIVITÉ BIOLOGIQUE DE PROTÉINES CHEZ LES VERTÉBRÉS, ET SES APPLICATIONS
Abstract:
(EN) The present invention relates to a method for tightly temporally controlling the biological activity of a protein of interest in a vertebrate, upon induction of the activity of a fusion protein comprising said protein of interest and an ERM polypeptide containing a mutated ligand binding domain of the human oestrogen receptor α, with a synthetic ligand that does not interfere with oestrogen signalling. In particular, the present invention concerns a method for generating tightly temporally-controlled targeted somatic mutations in a vertebrate, preferably a mouse, by inducing the activity of a fusion protein comprising a site- specific recombinase protein and an ERM polypeptide, with a synthetic ligand devoid of oestrogenic and anti-oestrogenic activities.
(FR) La présente invention concerne un procédé pour un contrôle temporaire ferme de l'activité biologique d'une protéine intéressante chez un vertébré, lors de l'induction de l'activité d'une protéine de fusion comprenant ladite protéine intéressante et un polypeptide ERM contenant un domaine de liaison au ligand muté du récepteur α des œstrogènes humains, celui-ci étant un ligand synthétique qui n'interfère pas avec la signalisation des œstrogènes. En particulier, la présente invention concerne un procédé pour générer des mutations somatiques ciblées avec un contrôle temporaire ferme chez un vertébré, de préférence une souris, en induisant l'activité d'une protéine de fusion comprenant une protéine recombinase régio-spécifique et un polypeptide ERM, avec un ligand synthétique dépourvu des activités œstrogénique et anti-œstrogénique.
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PE, PG, PH, PL, PT, RO, RS, RU, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)
Also published as:
EP2389391US20120052570CA2749789