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1. WO2010084015 - METHOD FOR DETECTING AN ANALYTE IN A SAMPLE BY MULTIPLEXING FRET ANALYSIS AND KIT

Publication Number WO/2010/084015
Publication Date 29.07.2010
International Application No. PCT/EP2010/000388
International Filing Date 22.01.2010
IPC
G01N 33/542 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
536with immune complex formed in liquid phase
542with steric inhibition or signal modification, e.g. fluorescent quenching
G01N 33/58 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
58involving labelled substances
CPC
B82Y 15/00
BPERFORMING OPERATIONS; TRANSPORTING
82NANOTECHNOLOGY
YSPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
15Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
G01N 33/542
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
536with immune complex formed in liquid phase
542with steric inhibition or signal modification, e.g. fluorescent quenching
G01N 33/588
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
58involving labelled substances
588with semiconductor nanocrystal label, e.g. quantum dots
Applicants
  • UNIVERSITÄT POTSDAM [DE]/[DE] (AllExceptUS)
  • CNRS [FR]/[FR] (AllExceptUS)
  • CEZANNE S.A.S. [FR]/[FR] (AllExceptUS)
  • HILDEBRANDT, Niko [DE]/[DE] (UsOnly)
  • GEIβLER, Daniel [DE]/[DE] (UsOnly)
  • LÖHMANNSRÖBEN, Hans-Gerd [DE]/[DE] (UsOnly)
  • BOIS, Emmanuel [FR]/[FR] (UsOnly)
  • CHARBONNIERE, Loïc [FR]/[FR] (UsOnly)
  • ZIESSEL, Raymond [FR]/[FR] (UsOnly)
Inventors
  • HILDEBRANDT, Niko
  • GEIβLER, Daniel
  • LÖHMANNSRÖBEN, Hans-Gerd
  • BOIS, Emmanuel
  • CHARBONNIERE, Loïc
  • ZIESSEL, Raymond
Agents
  • BITTNER, Thomas, L.
Priority Data
09000828.522.01.2009EP
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) METHOD FOR DETECTING AN ANALYTE IN A SAMPLE BY MULTIPLEXING FRET ANALYSIS AND KIT
(FR) PROCÉDÉ DE DÉTECTION D'UN ANALYTE DANS UN ÉCHANTILLON PAR MULTIPLEXAGE D'ANALYSE FRET ET KIT ASSOCIÉ
Abstract
(EN)
The invention concerns a method for detecting an analyte in a sample by multiplexing FRET (Förster Resonance Energy Transfer) analysis, the method comprising the following steps: providing a sample containing an energy transfer donor, several spectrally different quantum dot species, and an analyte, wherein the analyte is configured to mediate an energy transfer within a first energy transfer donor-acceptor pair provided by the energy transfer donor and a first quantum dot specie, the energy transfer donor is configured to act as energy transfer donor in the first energy transfer donor-acceptor pair, and the first quantum dot specie is configured to act as energy transfer acceptor in the first energy transfer donor-acceptor pair, irradiating excitation light from an excitation light source to the sample, in the first energy transfer donor-acceptor pair, transferring excitation energy from the energy transfer donor excited by the excitation light to the first quantum dot specie, the energy transfer being mediated by the analyte, and detecting emission light emitted by the first quantum dot specie after receiving the excitation energy.
(FR)
L'invention concerne un procédé de détection d'un analyte dans un échantillon par multiplexage d'analyse de transfert d'énergie par résonance de Förster (Förster Resonance Energy Transfer, FRET), le procédé comportant les étapes suivantes : mettre en place un échantillon contenant un donneur de transfert d'énergie, plusieurs espèces spectrales différentes de points quantiques et un analyte, l'analyte étant configuré pour servir d'intermédiaire pour un transfert d'énergie au sein d'une première paire donneur-accepteur de transfert d'énergie formée par le donneur de transfert d'énergie en question et une première espèce de points quantiques, le donneur de transfert d'énergie étant configuré pour agir comme donneur de transfert d'énergie dans la première paire donneur-accepteur de transfert d'énergie et la première espèce de points quantiques étant configurée pour agir comme accepteur de transfert d'énergie dans la première paire donneur-accepteur de transfert d'énergie, irradier une lumière d'excitation issue d'une source lumineuse d'excitation vers l'échantillon, dans la première paire donneur-accepteur de transfert d'énergie, transférer une énergie d'excitation issue du donneur de transfert d'énergie excité par la lumière d'excitation à la première espèce de points quantiques, le transfert d'énergie se faisant par l'intermédiaire de l'analyte, et détecter une lumière émise par la première espèce de points quantiques après qu'ils ont reçu l'énergie d'excitation.
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