Processing

Please wait...

Settings

Settings

1. WO2010082034 - ANTIBODY

Note: Text based on automatic Optical Character Recognition processes. Please use the PDF version for legal matters

Claims

1. A hybridoma deposited under accession number ECACC 08120202.

2. An antibody which may be obtained by culture of the hybridoma of claim 1 , or a functional fragment of such an antibody.

3. An antibody, or antibody fragment or other molecule capable of specifically binding to Aspergillus, that antibody, fragment or binding molecule comprising a CDR, light chain, heavy chain, light chain variable region, heavy chain variable region or antigen binding region, especially Fab region, that shows substantial homology with the corresponding region of the antibody according to the second aspect of the invention.

4. An antibody according to claim 3 comprising a CDR comprising an amino acid sequence having substantial homology to an amino acid sequence selected from the sequences shown in figures: 19 to 25.

5. An antibody according to claim 4, comprising a first CDR comprising an amino acid sequence having substantial homology to the amino acid sequence shown in figure:

19; a second CDR comprising an amino acid sequence having substantial homology to an amino acid sequence selected from the sequences shown in figures: 21 or 22; and a third CDR comprising an amino acid sequence having substantial homology to the amino acid sequence shown in figure: 24.

6. An antibody according to claim 4 comprising a first CDR comprising an amino acid sequence having substantial homology to the amino acid sequence shown in figure:

20; a second CDR comprising an amino acid sequence having substantial homology to the amino acid sequence shown in figure: 23; and a third CDR comprising an amino acid sequence having substantial homology to the amino acid sequence shown in figure: 25.

7. An antibody according to claim 3 comprising a heavy chain comprising one or more CDRs having an amino acid sequence having substantial homology to an amino acid sequence selected from the sequences shown in figures:

19, 21, 22 and 24.

8. An antibody according to claim 3 comprising a light chain comprising one or more CDRs having an amino acid sequence having substantial homology to an amino acid sequence selected from the sequences shown in figures:

20, 23 and 25.

9. An antibody according to claim 3comprising an amino acid sequence having substantial homology to an amino acid sequence selected from the sequences shown in figures:

2, 4, 6, 8, 10, 1 1, 13, 15, 17 and 18.

10. A n antibody according to claim 3 comprising a heavy chain variable region having substantial homology to an amino acid sequence selected from the sequences shown in figures: 2, 4, 6, 8, 10 and 1 1.

1 1. A n antibody according to claim 3 comprising a light chain variable region having substantial homology to the amino acid sequence selected from the sequences shown in figures:

13, 15, 17 and 18.

12. A n antibody according to claim 3 encoded by a nucleotide sequence having substantial homology to a nucleotide sequence selected from the sequences shown in figures:

1 , 3, 5, 7, 9, 12, H and 16.

13. A n antibody according to claim 3 comprising a heavy chain variable region encoded by a nucleotide sequence having substantial homology to a nucleotide sequence selected from the sequences shown in figures:

1 , 3, 5, 7 and 9.

14. A n antibody according to claim 3 comprising a light chain variable region encoded by a nucleotide sequence having substantial homology to the nucleotide sequence selected from the sequences shown in figures:

12, 14 and 16.

15. A n antibody that binds to the same epitope as an antibody according to any preceding claim.

16. A method of making antibodies, including a method of generating an antibody or functional fragment thereof comprising: a) providing a repertoire of nucleic acids encoding a variable domain that either includes a CDRl , CDR2 or CDR3 encoding region to be replaced or lacks a CDRl, CDR2 or CDR3 encoding region; b) combining the repertoire with a donor nucleic acid having a nucleotide sequence encoding a sequence selected from the sequences in figures:

19 to 25 to provide a repertoire of nucleic acids encoding a variable domain; and c) expressing a nucleic acid from the repertoire.

17. A n isolated nucleic acids encoding an antibody or functional fragment thereof according to any of claims 1 to 14.

18. A n isolated nucleic acid having substantial homology to a sequence selected from the sequences shown in figures:

1 , 3, 5, 7, 9, 12, H and 16.

19. A con struct comprising a nucleic acid molecule according to claim 18.

20. A host cell comprising at least one construct according to claim 19.

21. A the method of making an antibody comprising culturing a host cell according to claim 20 under appropriate conditions to express the antibody from the nucleic acid.

22. Al so provided is a method of assaying for the presence of an Aspergillus species in a sample, comprising: a) contacting the sample with labeled antibodies according to the invention; and b) observing the sample for binding of the antibodies to epitopes in the sample; wherein binding of the antibodies is indicative of the presence of an Aspergillus species.

23. A pharmaceutical composition comprising an antibody according to any of claims 1 to 14 or functional fragment thereof.

24. A n antibody according to any of claims 1 to 14 or functional fragment thereof according to the invention for use in therapy.

25. A method of treating an infection with an Aspergillus species, comprising administering a therapeutic amount of an antibody according to any of claims 1 to 14 or fragment thereof to a subject in need thereof.