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1. (WO2010080065) AFFINITY CHROMATOGRAPHY MATRIX
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/2010/080065 International Application No.: PCT/SE2010/050016
Publication Date: 15.07.2010 International Filing Date: 11.01.2010
IPC:
C07K 1/22 (2006.01) ,B01D 15/38 (2006.01) ,C12N 15/00 (2006.01) ,C07K 14/31 (2006.01) ,C07K 16/00 (2006.01)
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
1
General processes for the preparation of peptides
14
Extraction; Separation; Purification
16
by chromatography
22
Affinity chromatography or related techniques based upon selective absorption processes
B PERFORMING OPERATIONS; TRANSPORTING
01
PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
D
SEPARATION
15
Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
08
Selective adsorption, e.g. chromatography
26
characterised by the separation mechanism
38
involving specific interaction not covered by one or more of groups B01D15/30-B01D15/36120
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
14
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
195
from bacteria
305
from Micrococcaceae (F)
31
from Staphylococcus (G)
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
16
Immunoglobulins, e.g. monoclonal or polyclonal antibodies
Applicants:
GE HEALTHCARE BIO-SCIENCES AB [SE/SE]; Björkgatan 30 S-751 84 Uppsala, SE (AllExceptUS)
JOHANSSON, Hans, J. [SE/SE]; SE (UsOnly)
LJUNGLÖF, Anders [SE/SE]; SE (UsOnly)
PALMGREN, Ronnie [SE/SE]; SE (UsOnly)
Inventors:
JOHANSSON, Hans, J.; SE
LJUNGLÖF, Anders; SE
PALMGREN, Ronnie; SE
Agent:
GE HEALTHCARE BIO-SCIENCES AB; Patent Department Björkgatan 30 S-751 84 Uppsala, SE
Priority Data:
0900029-012.01.2009SE
Title (EN) AFFINITY CHROMATOGRAPHY MATRIX
(FR) MATRICE DE CHROMATOGRAPHIE PAR AFFINITÉ
Abstract:
(EN) The present invention relates to a method of separating one or more immunoglobulin containing proteins from a liquid. The method includes first contacting the liquid with a separation matrix comprising ligands immobilised to a support; allowing the immunoglobulin containing proteins to adsorb to the matrix by interaction with the ligands; followed by an optional step of washing the adsorbed immunoglobulin containing proteins; and recovering said immunoglobulin containing proteins by contacting the matrix with an eluent which releases the proteins. The method improves upon previous separation methods being that each of the ligands consists essentially of a monomer or dimer SpA or protein Z or a functional variant thereof.
(FR) La présente invention concerne un procédé de séparation d'une ou plusieurs protéines contenant des immunoglobulines à partir d'un liquide. Le procédé comprend les étapes suivantes: en premier lieu, mettre en contact le liquide avec une matrice de séparation comprenant des ligands immobilisés sur un support; permettre aux protéines contenant des immunoglobulines de s'adsorber sur la matrice par interaction avec les ligands; éventuellement, laver les protéines adsorbées contenant des immunoglobulines; et récupérer lesdites protéines contenant des immunoglobulines en mettant en contact la matrice avec un éluant qui libère les protéines. Le procédé permet une meilleure séparation que les procédés de séparation précédents puisque chacun des ligands consiste essentiellement en un monomère ou un dimère SpA ou une protéine Z ou un variant fonctionnel de ceux-ci.
front page image
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PE, PG, PH, PL, PT, RO, RS, RU, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)
Also published as:
EP2389386JP2012515160US20120149875CN102272145