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1. WO2010080032 - BEAD-ASSISTED VIRAL TRANSDUCTION

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Claims

1. Method of viral transduction of a cell in vitro, wherein a bead is coated with a virus and the virus coated bead is contacted with the cell to transfer a nucleic acid to the cell via the virus coating the bead, whereby the size of the bead is 2 to 20 micrometer.

2. Method according to claim 1, wherein the cell is freely floating in suspension.

3. Method according to claim 1 or 2, wherein the bead is precoated with a protein.

4. Method according to claim 3 wherein the bead is precoated with fibronectin, fragments or derivatives thereof.

5. Method according to any of claims 1 to 4, wherein the bead comprises an epoxy group, an maleimide group, an activated ester, or cyanogen bromide-activated group.

6. Method according to claim 5, wherein the activated ester is a N-hydroxysuccinimide ester.

7. Method according to any of claims 1 to 6, wherein the cell is a cell of the hematopoietic system.

8. Method according to any of claims 1 to 7, wherein the virus is a retrovirus, a lentivirus, an adenovirus, or an adeno-associated virus.

9. Virus coated bead for viral transduction of a cell according to the method of any of claims 1 to 8, whereby the size of the bead prior to coating is 2 to 20 micrometer.

10. Composition comprising the virus coated bead according to claim 9 and a cell, wherein the cell is freely floating in suspension.

11. Use of the virus coated bead according to claim 9, or the composition according to claim 10 for the preparation of a medicament for preventing and/or treating a disease which is responsive to the administration of a gene-modified cell.

12. Use according to claim 11, wherein the disease responsive to administration of a gene-modified cell is cancer, infection, immunodeficiency, storage disease, or autoimmunity.

13. Use of a bead with a size of 2 to 20 micrometer for viral transduction of a cell in vitro.

14. Use according to claim 13, wherein the size of the bead is 4.5 micrometer.

15. Use according to claim 13 or claim 14, wherein the bead is a polystyrene bead or a hydrophobic bead with glycidyl ether (epoxy) reactive groups.