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1. (WO2010078708) NEMATICIDAL BACILLUS THURINGIENSIS CRYSTAL PROTEIN GENE CRY1518-35
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/2010/078708 International Application No.: PCT/CN2009/001302
Publication Date: 15.07.2010 International Filing Date: 23.11.2009
IPC:
C12N 15/32 (2006.01) ,C07K 14/325 (2006.01) ,A01N 63/02 (2006.01) ,A01P 5/00 (2006.01) ,C12N 1/21 (2006.01) ,C12N 1/15 (2006.01) ,C12N 1/19 (2006.01) ,A01H 5/00 (2006.01) ,C12R 1/19 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
11
DNA or RNA fragments; Modified forms thereof
31
Genes encoding microbial proteins, e.g. enterotoxins
32
Bacillus crystal proteins
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
14
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
195
from bacteria
32
from Bacillus (G)
325
Bacillus thuringiensis crystal peptide (delta-endotoxin)
A HUMAN NECESSITIES
01
AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
N
PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
63
Biocides, pest repellants or attractants, or plant growth regulators containing micro-organisms, viruses, microbial fungi, animals, e.g. nematodes, or substances produced by, or obtained from micro-organisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
02
Substances produced by, or obtained from, micro-organisms or animals
A HUMAN NECESSITIES
01
AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
P
BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
5
Nematocides
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1
Micro-organisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving micro-organisms or compositions thereof; Processes of preparing or isolating a composition containing a micro-organism; Culture media therefor
20
Bacteria; Culture media therefor
21
modified by introduction of foreign genetic material
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1
Micro-organisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving micro-organisms or compositions thereof; Processes of preparing or isolating a composition containing a micro-organism; Culture media therefor
14
Fungi; Culture media therefor
15
modified by introduction of foreign genetic material
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1
Micro-organisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving micro-organisms or compositions thereof; Processes of preparing or isolating a composition containing a micro-organism; Culture media therefor
14
Fungi; Culture media therefor
16
Yeasts; Culture media therefor
19
modified by introduction of foreign genetic material
A HUMAN NECESSITIES
01
AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
H
NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
5
Flowering plants, i.e. angiosperms
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
R
INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C-C12Q75
1
Micro-organisms
01
Bacteria or actinomycetales
185
Escherichia
19
Escherichia coli
Applicants:
华中农业大学 HUAZHONG AGRICULTURAL UNIVERSITY [CN/CN]; 中国湖北省武汉市洪山区狮子山街1号 No. 1 Shizishan Street Hongshan District Wuhan Hubei 430070, CN (AllExceptUS)
孙明 SUN, Ming [CN/CN]; CN (UsOnly)
郭素霞 GUO, Suxia [CN/CN]; CN (UsOnly)
王鹏霞 WANG, Pengxia [CN/CN]; CN (UsOnly)
彭东海 PENG, Donghai [CN/CN]; CN (UsOnly)
阮丽芳 RUAN, Lifang [CN/CN]; CN (UsOnly)
喻子牛 YU, Ziniu [CN/CN]; CN (UsOnly)
Inventors:
孙明 SUN, Ming; CN
郭素霞 GUO, Suxia; CN
王鹏霞 WANG, Pengxia; CN
彭东海 PENG, Donghai; CN
阮丽芳 RUAN, Lifang; CN
喻子牛 YU, Ziniu; CN
Agent:
中国专利代理(香港)有限公司 CHINA PATENT AGENT (H.K.) LTD.; 中国香港特别行政区湾仔港湾道23号鹰君中心22号楼 22/F., Great Eagle Centre 23 Harbour Road Wanchai Hong Kong, CN
Priority Data:
200910060470.609.01.2009CN
Title (EN) NEMATICIDAL BACILLUS THURINGIENSIS CRYSTAL PROTEIN GENE CRY1518-35
(FR) GÈNE DE PROTÉINE CRISTAL DE BACILLUS THURINGIENSIS NÉMATICIDE CRY1518-35
(ZH) 苏云金芽胞杆菌杀线虫晶体蛋白基因CRY1518-35
Abstract:
(EN) Isolating, cloning and the biological activity to nematode of nematicidal Bacillus thuringiensis crystal protein gene are disclosed by the present invention. A novel nematicidal crystal protein gene cry1518-35 is isolated from the strain of Bacillus thuringiensis YBT-1518 which has insecticide activity to Meloidogyne hapla. The product encoded by said gene is a kind of novel nematicidal crystal protein Cry1518-35. Said protein has high insecticide activity to Meloidogyne hapla displayed by the biological assay.
(FR) La présente invention concerne l'isolement, le clonage et l'activité biologique sur des nématodes du gène de protéine cristal de Bacillus thuringiensis nématicide. Un nouveau gène de protéine cristal nématicide cry1518-35 a été isolé à partir de la souche de Bacillus thuringiensis YBT-1518 qui a une activité insecticide sur Meloidogyne hapla. Le produit codé par ledit gène est un type de nouvelle protéine cristal nématicide Cry1518-35. Ladite protéine a une activité insecticide élevée contre Meloidogyne hapla déterminée par un essai biologique.
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PE, PG, PH, PL, PT, RO, RS, RU, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG)
Publication Language: Chinese (ZH)
Filing Language: Chinese (ZH)