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1. (WO2010075632) METHODS FOR MULTIPLEX ANALYTE DETECTION AND QUANTIFICATION
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/2010/075632 International Application No.: PCT/CA2009/001899
Publication Date: 08.07.2010 International Filing Date: 29.12.2009
IPC:
G01N 33/53 (2006.01) ,C40B 30/04 (2006.01) ,G01N 33/543 (2006.01) ,G01N 33/564 (2006.01) ,G01N 33/58 (2006.01)
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53
Immunoassay; Biospecific binding assay; Materials therefor
C CHEMISTRY; METALLURGY
40
COMBINATORIAL TECHNOLOGY
B
COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICO LIBRARIES
30
Methods of screening libraries
04
by measuring the ability to specifically bind a target molecule, e.g. antibody-antigen binding, receptor-ligand binding
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53
Immunoassay; Biospecific binding assay; Materials therefor
543
with an insoluble carrier for immobilising immunochemicals
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53
Immunoassay; Biospecific binding assay; Materials therefor
564
for pre-existing immune complex or autoimmune disease
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
58
involving labelled substances
Applicants:
SQI DIAGNOSTICS SYSTEMS INC. [CA/CA]; 36 Meteor Drive Toronto, Ontario M9W 1A4, CA (AllExceptUS)
LEA, Peter [CA/CA]; CA (UsOnly)
Inventors:
LEA, Peter; CA
Agent:
KAPLAN, Adrian, M.; Heenan Blaikie LLP 333 Bay Street, Suite 2900 P.O. Box 2900 Bay Adelaide Centre Toronto, Ontario M5H 2T4, CA
Priority Data:
2,647,95329.12.2008CA
Title (EN) METHODS FOR MULTIPLEX ANALYTE DETECTION AND QUANTIFICATION
(FR) PROCÉDÉS DE DÉTECTION ET DE QUANTIFICATION MULTIPLEX D'ANALYTES
Abstract:
(EN) The application refers to a method for detecting and quantifying multiple target analytes in a test sample using a single reaction vessel. The method uses a reaction vessel (a multi-well plate), which comprises a microarray of : (a) calibration spots, each having a predetermined quantity of the target analyte; and (b) capture spots, each having an agent (antibody) that selectively binds the target analyte. The captured analytes and the calibration spots are detected with fluorescently labelled antibodies specific for each different target analyte. The calibration spots are used to generate calibration curves that allow the measurement of the concentration of the different target analytes. The application also refers to a method for detecting and quantifying biomarkers that are useful for diagnosing rheumatoid arthritis. More specifically, the application discloses the use of rheumatoid factor (RF) and cyclic citrullinated peptide (CCP), as capture spots. Finally, based on the above method, it is proposed a method for diagnosing or monitoring rheumatoid arthritis.
(FR) L'invention porte sur un procédé de détection et de quantification d'analytes cibles multiples dans un échantillon test à l'aide d'un seul récipient de réaction. Le procédé utilise un récipient de réaction (une plaque à puits multiples), qui comprend un microréseau de : (a) points d'étalonnage, chacun ayant une quantité prédéterminée de l'analyte cible ; et (b) points de capture, ayant chacun un agent (anticorps) qui se lie sélectivement à l'analyte cible. Les analytes capturés et les points d'étalonnage sont détectés par des anticorps à étiquetage fluorescent spécifiques pour chaque analyte cible différent. On utilise les points d'étalonnage pour générer des courbes d'étalonnage permettant la mesure de la concentration des différents analytes cibles. L'invention porte également sur un procédé de détection et de quantification de biomarqueurs qui sont utiles pour le diagnostic de la polyarthrite rhumatoïde. Plus spécifiquement, l'invention porte sur l'utilisation d'un facteur rhumatoïde (RF) et d'un peptide citrulliné cyclique (CCP) en tant que points de capture. Enfin, sur la base du procédé ci-dessus, on propose un procédé de diagnostic ou de surveillance de la polyarthrite rhumatoïde.
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PE, PG, PH, PL, PT, RO, RS, RU, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)
Also published as:
EP2382468JP2012514184US20110306511US20130165335CN102388306CA2748707
AU2009335612