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1. (WO2010075510) LARGE SCALE PANCREATIC ISLET PURIFICATION
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/2010/075510 International Application No.: PCT/US2009/069405
Publication Date: 01.07.2010 International Filing Date: 23.12.2009
Chapter 2 Demand Filed: 19.11.2010
IPC:
C12N 5/071 (2010.01) ,C12Q 1/24 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5
Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
07
Animal cells or tissues
071
Vertebrate cells or tissues, e.g. human cells or tissues
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
02
involving viable micro-organisms
24
Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact micro-organism
Applicants:
BAYLOR RESEARCH INSTITUTE [US/US]; 3434 Live Oak Street Suite 125 Dallas, TX 75204, US (AllExceptUS)
MATSUMOTO, Shinichi [JP/US]; US (UsOnly)
SHIMODA, Masayuki [JP/US]; US (UsOnly)
Inventors:
MATSUMOTO, Shinichi; US
SHIMODA, Masayuki; US
Agent:
FLORES, Edwin, S.; Chalker Flores LLP 2711 LBJ Freeway Suite 1036 Dallas, TX 75234 US, US
Priority Data:
61/140,91026.12.2008US
Title (EN) LARGE SCALE PANCREATIC ISLET PURIFICATION
(FR) PURIFICATION À GRANDE ÉCHELLE D'ÎLOTS PANCRÉATIQUES
Abstract:
(EN) The present invention includes a method of isolating pancreatic islets by density centrifugation wherein the pancreatic islets are loaded in a solution comprising pancreatic islets with the density gradient and the islets are isolated by centrifuging the vessel wherein the pressure on the pancreatic islets is less than 50Pa and wherein the pancreatic islets are isolated from the gradient, the improvement comprising creating a continuous density gradient in a vessel comprising at least 100 milliliters with a bend tube that reduces convection currents that disrupt the gradient.
(FR) La présente invention concerne un procédé d'isolement d'îlots pancréatiques utilisant la centrifugation par densité. Selon ledit procédé, les îlots pancréatiques sont chargés dans une solution comprenant les îlots pancréatiques présentant un gradient de densité; puis les îlots sont isolés en centrifugeant le récipient, la pression appliquée aux îlots pancréatiques étant inférieure à 50 Pa et les îlots pancréatiques étant isolés à partir du gradient. L'amélioration apportée par l'invention consiste en la création d'un gradient de densité continu dans un récipient comprenant au moins 100 millilitres et doté d'un tube coudé dont le rôle est de réduire les courants de convection qui perturbent le gradient.
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PE, PG, PH, PL, PT, RO, RS, RU, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)
Also published as:
IL213760EP2370565JP2012513755CN102317444CA2748267NZ593819
AU2009329910