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1. (WO2010075375) COMPOSITIONS, METHODS AND RELATED USES FOR CLEAVING MODIFIED DNA
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/2010/075375 International Application No.: PCT/US2009/069175
Publication Date: 01.07.2010 International Filing Date: 22.12.2009
IPC:
C12N 15/10 (2006.01) ,C12Q 1/68 (2006.01) ,C07K 16/00 (2006.01) ,C07K 14/00 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
10
Processes for the isolation, preparation or purification of DNA or RNA
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
68
involving nucleic acids
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
16
Immunoglobulins, e.g. monoclonal or polyclonal antibodies
C CHEMISTRY; METALLURGY
07
ORGANIC CHEMISTRY
K
PEPTIDES
14
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
Applicants:
NEW ENGLAND BIOLABS, INC. [US/US]; 240 County Road Ipswich, MA 01938, US (AllExceptUS)
ZHENG, Yu [US/US]; US (UsOnly)
ROBERTS, Richard, J. [GB/US]; US (UsOnly)
Inventors:
ZHENG, Yu; US
ROBERTS, Richard, J.; US
Agent:
STRIMPEL, Harriet, M.; New England Biolabs, Inc. 240 County Road Ipswich, MA 01938, US
Priority Data:
61/140,58623.12.2008US
61/267,61708.12.2009US
Title (EN) COMPOSITIONS, METHODS AND RELATED USES FOR CLEAVING MODIFIED DNA
(FR) COMPOSITIONS, PROCÉDÉS ET UTILISATIONS CORRESPONDANTES PERMETTANT DE COUPER L'ADN MODIFIÉ
Abstract:
(EN) Compositions, methods and related uses are provided relating to cleaving modified DNA. For example, a set of DNA fragments obtainable by enzymatic cleavage of a large DNA is described where at least 50% are similarly sized and have a centrally positioned modified nucleotide. In addition, an enzyme preparation is provided that includes one or more enzymes that recognize a modified nucleotide in a DNA and cleave the DNA at a site that is at a non-random distance from the modified nucleotide. The one or more enzymes are further characterized by an N- terminal conserved domain with greater than 90% amino acid sequence homology to WXD(X)10YXGD. The related uses include creating a methylome, methods of purifying DNA fragments containing a modified nucleotide and diagnostic applications.
(FR) La présente invention concerne des compositions, des procédés et des utilisations correspondantes permettant de couper l'ADN modifié. L'invention concerne notamment l'obtention d'un ensemble de fragments d'ADN par coupure enzymatique d'un grand ADN, fragments dont environ 50% sont de dimensions semblables et comportent en position centrale un nucléotide modifié. L'invention concerne également une préparation d'enzyme comportant un ou plusieurs enzymes qui reconnaissent un nucléotide modifié dans un ADN et coupent l'ADN au niveau d'un site qui se situe à une distance non aléatoire par rapport au nucléotide modifié. Le ou les enzymes considérés sont en outre caractérisés par un domaine conservé de la terminaison N- présentant une homologie à plus de 90% entre la séquence d'acides aminés et WXD(X)10YXGD. Les utilisations prises en considération ici concernent la création d'un méthylome, des procédés de purification de fragments d'ADN contenant un nucléotide modifié, et des applications de diagnostic.
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PE, PG, PH, PL, PT, RO, RS, RU, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)
Also published as:
EP2376632JP2013514758CN102264900IN4043/CHENP/2011