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1. (WO2010075194) PROKARYOTIC EXPRESSION OF SOLUBLE, ACTIVE DKK
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/2010/075194 International Application No.: PCT/US2009/068628
Publication Date: 01.07.2010 International Filing Date: 18.12.2009
IPC:
C12P 21/00 (2006.01) ,C12N 1/21 (2006.01) ,C12N 15/23 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
P
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
21
Preparation of peptides or proteins
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1
Micro-organisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving micro-organisms or compositions thereof; Processes of preparing or isolating a composition containing a micro-organism; Culture media therefor
20
Bacteria; Culture media therefor
21
modified by introduction of foreign genetic material
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
11
DNA or RNA fragments; Modified forms thereof
12
Genes encoding animal proteins
19
Interferons; Lymphokines; Cytokines
20
Interferons
23
Gamma-interferons
Applicants:
ST. JUDE CHILDREN'S RESEARCH HOSPITAL [US/US]; 332 North Lauderdale Street Memphis, TN 38105-2794, US (AllExceptUS)
ZHENG, Jie [US/US]; US (UsOnly)
Inventors:
ZHENG, Jie; US
Agent:
LICATA, Jane Massey; Licata & Tyrrell P.C. 66 E. Main Street Marlton, NJ 08053, US
Priority Data:
61/139,71122.12.2008US
Title (EN) PROKARYOTIC EXPRESSION OF SOLUBLE, ACTIVE DKK
(FR) EXPRESSION PROCARYOTE DE DKK SOLUBLE ACTIVE
Abstract:
(EN) Dickkopf (Dkk) proteins inhibit the canonical Wnt signaling pathway. Each of the members of the Dkk family has been previously cloned and expressed as a soluble protein in eukaryotic cells, while expression in bacterial cells has resulted in the formation of insoluble inclusion bodies that require further processing. The present invention provides compositions and methods for producing soluble, active dkk protein in prokaryotic host cells, by expressing the dkk protein as a fusion protein with a solubilization molecule, thereby providing an inexpensive and convenient source of pure active Dkk.
(FR) Les protéines de Dickkopf (Dkk) inhibent la voie de signalisation canonique des protéines Wnt. Chacun des membres de la famille des Dkk a été précédemment cloné et exprimé sous forme d'une protéine soluble dans des cellules eucaryotes, tandis que l'expression dans des cellules bactériennes a entraîné la formation de corps d'inclusion insolubles nécessitant un traitement ultérieur. La présente invention concerne des compositions et des méthodes de production de protéines Dkk solubles actives dans des cellules hôtes procaryotes par expression de la protéine Dkk sous forme d'une protéine de fusion avec une molécule de solubilisation, ce qui permet d'obtenir une source pratique et peu onéreuse de Dkk active pure.
front page image
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PE, PG, PH, PL, PT, RO, RS, RU, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)