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1. (WO2010074209) MUTANT LIPASE AND USE THEREOF
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/2010/074209 International Application No.: PCT/JP2009/071553
Publication Date: 01.07.2010 International Filing Date: 25.12.2009
IPC:
C12N 15/09 (2006.01) ,C12N 1/21 (2006.01) ,C12N 9/20 (2006.01) ,C12P 7/20 (2006.01) ,C12P 13/08 (2006.01) ,C12P 13/22 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1
Micro-organisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving micro-organisms or compositions thereof; Processes of preparing or isolating a composition containing a micro-organism; Culture media therefor
20
Bacteria; Culture media therefor
21
modified by introduction of foreign genetic material
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9
Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
14
Hydrolases (3.)
16
acting on ester bonds (3.1)
18
Carboxylic ester hydrolases
20
Triglyceride splitting, e.g. by means of lipase
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
P
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
7
Preparation of oxygen-containing organic compounds
02
containing a hydroxy group
04
acyclic
18
polyhydric
20
Glycerol
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
P
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
13
Preparation of nitrogen-containing organic compounds
04
Alpha- or beta-amino acids
08
Lysine; Diaminopimelic acid; Threonine; Valine
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
P
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
13
Preparation of nitrogen-containing organic compounds
04
Alpha- or beta-amino acids
22
Tryptophan; Tyrosine; Phenylalanine; 3,4-Dihydroxyphenylalanine
Applicants:
味の素株式会社 AJINOMOTO CO., INC. [JP/JP]; 東京都中央区京橋一丁目15番1号 15-1, Kyobashi 1-chome, Chuo-ku, Tokyo 1048315, JP (AllExceptUS)
独立行政法人酒類総合研究所 NATIONAL RESEARCH INSTITUTE OF BREWING [JP/JP]; 広島県東広島市鏡山三丁目7番1号 7-1, Kagamiyama 3-chome, Higashihiroshima-shi, Hiroshima 7390046, JP (AllExceptUS)
中田 國夫 NAKATA, Kunio [JP/JP]; JP (UsOnly)
臼田 佳弘 USUDA, Yoshihiro [JP/JP]; JP (UsOnly)
榛葉 信久 SHIMBA, Nobuhisa [JP/JP]; JP (UsOnly)
星野 亘 HOSHINO, Wataru [JP/JP]; JP (UsOnly)
鈴木 榮一郎 SUZUKI, Eiichiro [JP/JP]; JP (UsOnly)
家藤 治幸 IEFUJI, Haruyuki [JP/JP]; JP (UsOnly)
正木 和夫 MASAKI, Kazuo [JP/JP]; JP (UsOnly)
Inventors:
中田 國夫 NAKATA, Kunio; JP
臼田 佳弘 USUDA, Yoshihiro; JP
榛葉 信久 SHIMBA, Nobuhisa; JP
星野 亘 HOSHINO, Wataru; JP
鈴木 榮一郎 SUZUKI, Eiichiro; JP
家藤 治幸 IEFUJI, Haruyuki; JP
正木 和夫 MASAKI, Kazuo; JP
Agent:
川口 嘉之 KAWAGUCHI, Yoshiyuki; 東京都中央区東日本橋3丁目4番10号 アクロポリス21ビル6階 Acropolis 21 Building 6th floor, 4-10, Higashi Nihonbashi 3-chome, Chuo-ku, Tokyo 1030004, JP
Priority Data:
2008-33177126.12.2008JP
Title (EN) MUTANT LIPASE AND USE THEREOF
(FR) LIPASE MUTANTE ET UTILISATION ASSOCIÉE
(JA) 変異型リパーゼとその応用
Abstract:
(EN) A mutant lipase produced by introducing a mutation into a lipase produced by a microorganism belonging to the genus Cryptococcus, Gibberella, Ustilago or the like so as to increase the stability of the enzyme. An L-amino acid such as L-lysine, L-threonine or L-tryptophan can be produced through a fermentation process using, as a carbon source, glycerol or the like that is produced by reacting the mutant lipase with an oil-and-fat.
(FR) La présente invention a pour objet une lipase mutante produite par l'introduction d'une mutation dans une lipase produite par un micro-organisme appartenant au genre Cryptococcus, Gibberella, Ustilago ou analogue, de sorte à augmenter la stabilité de l'enzyme. Un acide L-aminé tel que la L-lysine, la L-thréonine ou le L-tryptophane peut être produit grâce à un processus de fermentation utilisant, en tant que source de carbone, du glycérol ou analogue qui est produit par la mise en réaction de la lipase mutante avec un produit d'huile et de graisse.
(JA) クリプトコッカス属、ジベレラ属、またはウスチラゴ属等の微生物が産生するリパーゼに酵素の安定性が上昇する変異を導入した変異型リパーゼを油脂に作用させて、グリセロール等を生成させ、それを炭素源としてL-リジン、L-スレオニン、及びL-トリプトファン等のL-アミノ酸を発酵法により製造する。
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PE, PG, PH, PL, PT, RO, RS, RU, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG)
Publication Language: Japanese (JA)
Filing Language: Japanese (JA)