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1. (WO2010073972) METHOD FOR OBTAINING PANCREATIC PROGENITOR CELL USING NEPH3
Latest bibliographic data on file with the International Bureau
Pub. No.: WO/2010/073972 International Application No.: PCT/JP2009/071089
Publication Date: 01.07.2010 International Filing Date: 18.12.2009
IPC:
C12N 5/071 (2010.01) ,G01N 33/53 (2006.01) ,C12N 15/09 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5
Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
07
Animal cells or tissues
071
Vertebrate cells or tissues, e.g. human cells or tissues
G PHYSICS
01
MEASURING; TESTING
N
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33
Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48
Biological material, e.g. blood, urine; Haemocytometers
50
Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53
Immunoassay; Biospecific binding assay; Materials therefor
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
Applicants:
エーザイ・アール・アンド・ディー・マネジメント株式会社 EISAI R&D MANAGEMENT CO., LTD. [JP/JP]; 東京都文京区小石川四丁目６番１０号 6-10, Koishikawa 4-chome, Bunkyo-ku, Tokyo 1128088, JP (AllExceptUS)
尾野　雄一 ONO, Yuichi [JP/JP]; JP (UsOnly)
中谷　智哉 NAKATANI, Tomoya [JP/JP]; JP (UsOnly)
中川　康子 NAKAGAWA, Yasuko [JP/JP]; JP (UsOnly)
Inventors:
尾野　雄一 ONO, Yuichi; JP
中谷　智哉 NAKATANI, Tomoya; JP
中川　康子 NAKAGAWA, Yasuko; JP
Agent:
清水　初志 SHIMIZU, Hatsushi; 茨城県土浦市卸町１－１－１　関鉄つくばビル６階 Kantetsu Tsukuba Bldg. 6F, 1-1-1, Oroshi-machi, Tsuchiura-shi, Ibaraki 3000847, JP
Priority Data:
2008-32640122.12.2008JP
2009-04066724.02.2009JP
Title (EN) METHOD FOR OBTAINING PANCREATIC PROGENITOR CELL USING NEPH3
(FR) PROCÉDÉ D'OBTENTION D'UNE CELLULE PROGÉNITRICE PANCRÉATIQUE À L'AIDE DE NEPH3
(JA) Ｎｅｐｈ３を用いた膵臓前駆細胞の取得方法
Abstract:
(EN) Disclosed is a marker capable of identifying a pancreatic progenitor cell selectively. Also disclosed is a method for identifying a pancreatic progenitor cell using the maker as an indicator. Further disclosed is a reagent for use in the method. A surface marker Neph3 (Nephrin-like 3) capable of being expressed in a pancreatic progenitor cell specifically is identified, and the isolation or the like of a pancreatic progenitor cell is successfully achieved using the marker as an indicator. The use of Neph3 as an indicator enables the selection of a pancreatic progenitor cell alive without the need of using any translation product or transcription product of a foreign gene. The marker is useful for obtaining or identifying a pancreatic progenitor cell for use in regenerative medicine for pancreatic diseases or the like.
(FR) L'invention porte sur un marqueur capable d'identifier une cellule progénitrice pancréatique de manière sélective. L'invention porte également sur un procédé d'identification d'une cellule progénitrice pancréatique à l'aide du marqueur en tant qu'indicateur. L'invention porte en outre sur un réactif pour une utilisation dans le procédé. Un marqueur de surface Neph3 (de type néphrine 3) capable d'être exprimé de manière spécifique dans une cellule progénitrice pancréatique est identifié, et l'isolement ou similaire d'une cellule progénitrice pancréatique est obtenu avec succès à l'aide du marqueur en tant qu'indicateur. L'utilisation de Neph3 en tant qu'indicateur permet la sélection d'une cellule progénitrice pancréatique vivante sans utiliser un quelconque produit de traduction ou produit de transcription d'un gène étranger. Le marqueur est utile pour obtenir ou identifier une cellule progénitrice pancréatique pour une utilisation en médecine régénérative pour des maladies pancréatiques ou similaires.
(JA) 【課題】本発明は、膵臓前駆細胞を選択的に識別可能なマーカーを提供する。また本発明は、該マーカーを指標として膵臓前駆細胞を識別する方法、並びに、該方法に用いるための試薬を提供する。 【解決手段】　膵臓前駆細胞で特異的に発現する表面マーカーNeph3(Nephrin-like 3)を同定し、該マーカーを指標として膵臓前駆細胞を分離等することに成功した。Neph3を指標とすることで、外来遺伝子の翻訳産物や転写産物を用いることなく膵臓前駆細胞を生きたまま選別することが可能である。同マーカーは、膵臓疾患の再生医療等に適用するための膵臓前駆細胞の取得や同定に有用である。
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PE, PG, PH, PL, PT, RO, RS, RU, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG)
Publication Language: Japanese (JA)
Filing Language: Japanese (JA)
Also published as:
EP2377921JPWO2010073972US20110256548