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1. (WO2010073808) METHOD FOR WASHING CELLS
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/2010/073808 International Application No.: PCT/JP2009/068237
Publication Date: 01.07.2010 International Filing Date: 23.10.2009
IPC:
C12N 5/07 (2010.01) ,C12N 1/02 (2006.01) ,B04B 11/04 (2006.01) ,B04B 15/12 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5
Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
07
Animal cells or tissues
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1
Micro-organisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving micro-organisms or compositions thereof; Processes of preparing or isolating a composition containing a micro-organism; Culture media therefor
02
Separating micro-organisms from their culture media
B PERFORMING OPERATIONS; TRANSPORTING
04
CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
B
CENTRIFUGES
11
Feeding, charging, or discharging bowls
04
Periodical feeding or discharging; Control arrangements therefor
B PERFORMING OPERATIONS; TRANSPORTING
04
CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
B
CENTRIFUGES
15
Other accessories for centrifuges
12
for drying or washing the separated solid particles
Applicants:
オリンパス株式会社 OLYMPUS CORPORATION [JP/JP]; 東京都渋谷区幡ヶ谷2丁目43番2号 43-2, Hatagaya 2-chome, Shibuya-ku, Tokyo 1510072, JP (AllExceptUS)
サイトリ セラピューティクス インコーポレイテッド Cytori Therapeutics, Inc. [US/US]; カリフォルニア州サンディエゴ カランロード 3020 3020, Callan Road, San Diego, California 92121, US (AllExceptUS)
土田 裕基 TSUCHIDA, Hiroki [JP/JP]; JP (UsOnly)
唐澤 千里 KARASAWA, Chisato [JP/JP]; JP (UsOnly)
吉田 和博 YOSHIDA, Kazuhiro [JP/JP]; JP (UsOnly)
シャナハン ロバート ケイ SHANAHAN, Robert K. [US/US]; US (UsOnly)
フォルナーチェ ルーカス ヴイ FORNACE, Lucas V. [US/US]; US (UsOnly)
Inventors:
土田 裕基 TSUCHIDA, Hiroki; JP
唐澤 千里 KARASAWA, Chisato; JP
吉田 和博 YOSHIDA, Kazuhiro; JP
シャナハン ロバート ケイ SHANAHAN, Robert K.; US
フォルナーチェ ルーカス ヴイ FORNACE, Lucas V.; US
Agent:
上田 邦生 UEDA, Kunio; 神奈川県横浜市西区みなとみらい2-2-1 横浜ランドマークタワー37F 37F The Landmark Tower Yokohama, 2-2-1, Minatomirai, Nishi-ku, Yokohama-shi, Kanagawa 2208137, JP
Priority Data:
2008-33103125.12.2008JP
Title (EN) METHOD FOR WASHING CELLS
(FR) PROCÉDÉ DE LAVAGE DE CELLULES
(JA) 細胞洗浄方法
Abstract:
(EN) By efficiently washing cells, cells from which unnecessary components have been removed are collected.  A method for washing cells which comprises: a centrifugation step (S1) comprising introducing a cell suspension into a centrifuge container provided with a washing liquor-jetting port facing the bottom, and rotating the container in such a manner that the bottom is radially outwardly directed, to thereby centrifuge and sediment the cells at the bottom; a supernatant-discharging step (S2) for discharging the supernatant separated by the centrifugation in the preceding centrifugation step (S1); and a washing step (S3) for, after discharging the supernatant in the preceding supernatant-discharging step (S2), jetting a washing liquor from the jetting port while rotating the centrifuge container.
(FR) La présente invention concerne un procédé efficace de lavage de cellules qui permet de collecter des cellules dont les composants inutiles ont été éliminés. Ce procédé comprend une étape de centrifugation (S1) qui consiste à introduire une suspension de cellules dans une centrifugeuse dont le fond est pourvu d'un orifice d'injection d'eau de lavage et faire tourner cette centrifugeuse de manière à ce que le fond soit dirigé radialement vers l'extérieur, pour centrifuger et sédimenter les cellules dans le fond ; une étape de retrait du surnageant (S2) qui consiste à retirer le surnageant séparé par centrifugation à l'étape (S1) ; et une étape de lavage (S3) qui consiste, après l'étape (S2), à injecter une eau de lavage par l'orifice d'injection tout en faisant tourner la centrifugeuse.
(JA)  細胞群を効率的に洗浄して不要成分が除去された細胞群を回収する。底部に対向した洗浄液の吐出口を有する遠心容器に細胞懸濁液を収容し、底部が半径方向外方に向かうように回転させて細胞を底部に遠心分離する遠心分離工程(S1)と、該遠心分離工程(S1)において遠心分離された上清を排出する上清排出工程(S2)と、該上清排出工程(S2)において上清を排出した状態で、遠心容器を回転させた状態で、吐出口から洗浄液を吐出する洗浄工程(S3)とを含む細胞洗浄方法を提供する。
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PE, PG, PH, PL, PT, RO, RS, RU, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG)
Publication Language: Japanese (JA)
Filing Language: Japanese (JA)
Also published as:
EP2371943