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1. (WO2010072118) PROBES, LIQUID PHASE CHIPS AND METHODS FOR DETECTING PIK3CA GENE MUTATIONS
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/2010/072118 International Application No.: PCT/CN2009/075451
Publication Date: 01.07.2010 International Filing Date: 10.12.2009
IPC:
C12N 15/11 (2006.01) ,C12Q 1/68 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
11
DNA or RNA fragments; Modified forms thereof
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
68
involving nucleic acids
Applicants:
广州益善生物技术有限公司 GUANGZHOU SUREXAM BIO-TECH CO., LTD. [CN/CN]; 中国广东省广州市 科学城揽月路80号广州科技创新基地B、C区五层 5/F, Block B&C, Guangzhou Technology Innovation Base, No.80, Lanyue Road, Science City Guangzhou, Guangdong 510663, CN (AllExceptUS)
许嘉森 XU, Jiasen [CN/CN]; CN (UsOnly)
郭元杰 GUO, Yuanjie [CN/CN]; CN (UsOnly)
Inventors:
许嘉森 XU, Jiasen; CN
郭元杰 GUO, Yuanjie; CN
Agent:
广州华进联合专利商标代理有限公司 ADVANCE CHINA I. P. LAW OFFICE; 中国广东省广州市 先烈中路69号东山广场918-920室万志香 WAN, Zhixiang Suite 918-920, 9/F., Dong Shan Plaza, NO.69 Xianlie Central Road Guangzhou, Guangdong 510095, CN
Priority Data:
200810220266.123.12.2008CN
Title (EN) PROBES, LIQUID PHASE CHIPS AND METHODS FOR DETECTING PIK3CA GENE MUTATIONS
(FR) SONDES, PUCES EN PHASE LIQUIDE ET PROCÉDÉS DE DÉTECTION DE MUTATIONS DU GÈNE PIK3CA
(ZH) PIK3CA基因突变的检测探针、液相芯片及其检测方法
Abstract:
(EN) Probes, liquid phase chips and methods for detecting PIK3CA gene mutations are provided, wherein the liquid phase chips for detecting PIK3CA gene mutations mainly comprise: microspheres enveloped with probes; primers used for amplifying target sequences with 9 exon and/or 20 exon. The liquid phase chips and methods for detecting PIK3CA gene mutations are useful for detecting the sites containing mutations of the PIK3CA gene with relatively high frequency simultaneously, and also are useful for detecting the exon 9 and exon 20 separately or simultaneously. The detection methods have identical reaction conditions of detection, good specificity of detection, high sensitivity, above 90% accuracy, and short time of detection.
(FR) La présente invention concerne des sondes, des puces en phase liquide et des procédés de détection de mutations du gène PIK3CA, lesdites puces en phase liquide destinées à la détection de mutations du gène PIK3CA comprenant : des microsphères enveloppées par des sondes ; des amorces utilisées pour amplifier des séquences cibles comportant l'exon 9 et/ou l'exon 20. Les puces en phase liquide et les procédés de détection de mutations du gène PIK3CA sont utiles pour détecter les sites contenant des mutations du gène PIK3CA avec une fréquence relativement élevée simultanément, et sont également utiles pour détecter l'exon 9 et l'exon 20 séparément ou simultanément. Les procédés de détection ont des conditions réactionnelles de détection identiques, une bonne spécificité de détection, une sensibilité élevée, une exactitude supérieure à 90 %, et un temps de détection court.
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PE, PG, PH, PL, PT, RO, RS, RU, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG)
Publication Language: Chinese (ZH)
Filing Language: Chinese (ZH)
Also published as:
EP2377933US20110312523