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1. WO2010033722 - CONSTRUCTION OF FULLY-DELETED ADENOVIRUS-BASED GENE DELIVERY VECTORS AND USES THEREOF

Publication Number WO/2010/033722
Publication Date 25.03.2010
International Application No. PCT/US2009/057344
International Filing Date 17.09.2009
IPC
C12N 5/00 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
A01N 63/00 2006.1
AHUMAN NECESSITIES
01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
63Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
A61K 48/00 2006.1
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
48Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
CPC
C12N 15/86
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
85for animal cells
86Viral vectors
C12N 2710/10043
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
2710dsDNA Viruses
00011dsDNA Viruses
10011Adenoviridae
10041Use of virus, viral particle or viral elements as a vector
10043viral genome or elements thereof as genetic vector
C12N 2710/10052
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
2710dsDNA Viruses
00011dsDNA Viruses
10011Adenoviridae
10051Methods of production or purification of viral material
10052relating to complementing cells and packaging systems for producing virus or viral particles
C12N 2710/10343
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
2710dsDNA Viruses
00011dsDNA Viruses
10011Adenoviridae
10311Mastadenovirus, e.g. human or simian adenoviruses
10341Use of virus, viral particle or viral elements as a vector
10343viral genome or elements thereof as genetic vector
C12N 2710/10352
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
2710dsDNA Viruses
00011dsDNA Viruses
10011Adenoviridae
10311Mastadenovirus, e.g. human or simian adenoviruses
10351Methods of production or purification of viral material
10352relating to complementing cells and packaging systems for producing virus or viral particles
C12N 7/00
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
7Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
Applicants
  • ISOGENIS, INC. [US]/[US] (AllExceptUS)
  • BRENNAN, Miles, B. [US]/[US] (UsOnly)
  • SPIEGEL, Erin, K. [US]/[US] (UsOnly)
  • STAERZ, Uwe, D. [US]/[US] (UsOnly)
  • WALL, Charles [US]/[US] (UsOnly)
  • WHEELER, Janae [US]/[US] (UsOnly)
  • MASLANIK, William, J. [US]/[US] (UsOnly)
  • ZHANG, Xianghua [US]/[US] (UsOnly)
Inventors
  • BRENNAN, Miles, B.
  • SPIEGEL, Erin, K.
  • STAERZ, Uwe, D.
  • WALL, Charles
  • WHEELER, Janae
  • MASLANIK, William, J.
  • ZHANG, Xianghua
Agents
  • ABRAMSON, Danielle, T.
Priority Data
61/097,73517.09.2008US
61/143,28108.01.2009US
61/236,57725.08.2009US
Publication Language English (en)
Filing Language English (EN)
Designated States
Title
(EN) CONSTRUCTION OF FULLY-DELETED ADENOVIRUS-BASED GENE DELIVERY VECTORS AND USES THEREOF
(FR) CONSTRUCTION DE VECTEURS DE DÉLIVRANCE DE GÈNES BASÉS SUR UN ADÉNOVIRUS COMPLÈTEMENT DÉLÉTÉ ET SES UTILISATIONS
Abstract
(EN) The embodiments disclosed herein relate to the construction of fully-deleted Adenovirus-based gene delivery vectors packaged without helper Adenovirus, and more particularly to their use in gene therapy for gene and protein expression, vaccine development, and immunosuppressive therapy for allogeneic transplantation. In an embodiment, a method for propagating an adenoviral vector includes (a) providing an Adenovirus packaging cell line; (b) transfecting a fully-deleted Adenoviral vector construct into the cell line; and optionally (c) transfecting a packaging construct into the cell line, wherein the fully-deleted Adenoviral vector construct and optionally the packaging construct can transfect the Adenovirus packaging cell line resulting in the encapsidation of a fully-deleted Adenoviral vector independent of helper Adenovirus. In an embodiment, a target cell is transduced with the encapsidated fully-deleted Adenoviral vector for treating a condition, disease or a disorder.
(FR) Les modes de réalisation de l'invention décrits ici concernent la construction de vecteurs de délivrance de gènes basés sur un adénovirus complètement délété empaquetés sans adénovirus auxiliaire, et plus particulièrement leur utilisation en thérapie génique pour l'expression de gènes et de protéines, le développement de vaccins, et la thérapie immunosuppressive pour allotransplantation. Dans un mode de réalisation, un procédé de propagation d'un vecteur adénoviral comprend (a) l'obtention d'une lignée cellulaire d'empaquetage d'adénovirus; (b) la transfection d'une construction de vecteur adénoviral complètement délété; et en option (c) la transfection d'une construction d'empaquetage dans la lignée cellulaire, la construction de vecteur adénoviral entièrement délété et en option la construction d'empaquetage pouvant transfecter la lignée cellulaire d'empaquetage d'adénovirus en permettant l'encapsidation d'un vecteur adénoviral entièrement délété indépendamment d'un adénovirus auxiliaire. Dans un mode de réalisation, une cellule cible est transduite avec le vecteur adénoviral entièrement délété encapsidé pour traiter un état, une maladie ou une affection.
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