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Pub. No.:    WO/2010/033652    International Application No.:    PCT/US2009/057233
Publication Date: 25.03.2010 International Filing Date: 17.09.2009
C12Q 1/68 (2006.01), C07H 21/02 (2006.01)
Applicants: GE HEALTHCARE BIO-SCIENCES CORP. [US/US]; 800 Centennial Avenue Piscataway, New Jersey 08855 (US) (For All Designated States Except US).
DHULIPALA, Rohini [US/US]; (US) (For US Only).
CAI, Yuyang Christine [US/US]; (US) (For US Only).
JIANG, Miao [CN/US]; (US) (For US Only).
DAR, Mubasher [US/US]; (US) (For US Only)
Inventors: DHULIPALA, Rohini; (US).
CAI, Yuyang Christine; (US).
JIANG, Miao; (US).
DAR, Mubasher; (US)
Agent: JI, Yonggang; (US)
Priority Data:
61/097,604 17.09.2008 US
61/148,126 29.01.2009 US
Abstract: front page image
(EN)This invention relates to a simple and rapid method for the extraction and purification of small RNA from a sample solution. Accordingly, a sample is first mixed with an organic solvent to form a mixture containing the solvent. The mixture is applied to a first mineral support for large RNA to bind. The filtrate is collected which contain unbound small RNA, and is mixed with a second organic solvent to form a second mixture containing the second solvent. This second mixture is applied to a second mineral support for small RNA to bind. After a wash step, the small RNA is eluted. Also provided is a method for the isolation of large RNA, by eluting the large RNA from the first mineral support. In addition, total protein is present in the filtrate and can be isolated by a conventional method.
(FR)L'invention concerne un procédé simple et rapide pour l'extraction et la purification d'un petit ARN à partir d'une solution d'échantillon. Un échantillon est tout d'abord mélangé avec un solvant organique pour former un mélange contenant le solvant. Le mélange est appliqué à un premier support minéral auquel le grand ARN se lie. Le filtrat est recueilli. Il contient le petit ARN non lié, et est mélangé avec un second solvant organique pour former un second mélange contenant le second solvant. Ce second mélange est appliqué à un second support minéral auquel le petit ARN se lie. Après une étape de lavage, le petit ARN est élué. L'invention concerne également un procédé d'isolement d'un grand ARN, par élution du grand ARN du premier support minéral. En outre, une protéine totale est présente dans le filtrat et peut être isolée par un procédé classique.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PE, PG, PH, PL, PT, RO, RS, RU, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW.
African Regional Intellectual Property Organization (BW, GH, GM, KE, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG).
Publication Language: English (EN)
Filing Language: English (EN)