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Machine translation
1. (WO2010030608) METHODS FOR DETECTING AND QUANTIFYING OVERSULFATED GLYCOSAMINOGLYCANS
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/2010/030608    International Application No.:    PCT/US2009/056263
Publication Date: 18.03.2010 International Filing Date: 08.09.2009
IPC:
C12Q 1/68 (2006.01), C12Q 1/48 (2006.01), G01N 33/86 (2006.01)
Applicants: THE GOVERNMENT OF THE UNITED STATES OF AMERICA AS REPRESENTED BY THE SECRETARY OF THE DEPARTMENT OF HEALTH AND HUMAN SERVICES [US/US]; National Institutes of Health Office of Technology Transfer 6011 Executive Blvd, Suite 325 MSC 7660 Bethesda, MD 20892-7660 (US) (For All Designated States Except US).
VERTHELYI, Daniela [US/US]; (US) (For US Only).
TAMI, Maria, Cecilia [AR/US]; (US) (For US Only).
PUIG, Montserrat [ES/US]; (US) (For US Only)
Inventors: VERTHELYI, Daniela; (US).
TAMI, Maria, Cecilia; (US).
PUIG, Montserrat; (US)
Agent: HARDING, Tanya, M.; (US)
Priority Data:
61/095,562 09.09.2008 US
Title (EN) METHODS FOR DETECTING AND QUANTIFYING OVERSULFATED GLYCOSAMINOGLYCANS
(FR) PROCÉDÉS DE DÉTECTION ET DE QUANTIFICATION DE GLYCOSAMINOGLYCANES SUR-SULFATÉS
Abstract: front page image
(EN)The invention relates to novel methods for detecting and/or quantifying oversulfated or persulfated glycosaminoglycans based on inhibition of nucleic acid polymerases. The methods can be utilized to detect and quantify oversulfated or persulfated glycosaminoglycans in pharmaceutical preparations, such as heparin preparations or therapeutic medical devices. When used to detect or quantify oversulfated glycosaminoglycans in heparin containing solutions, the samples are prepared by treatment with heparinases to degrade the heparin. Titration of the inhibition of the activity of the polymerases allows quantitation of the oversulfated glycosaminoglycans in the sample.
(FR)La présente invention concerne de nouveaux procédés de détection et/ou de quantification de glycosaminoglycanes sur-sulfatés ou persulfatés se basant sur l’inhibition d'acides nucléiques polymérases. Les procédés peuvent être utilisés pour détecter et quantifier des glycosaminoglycanes sur-sulfatés ou persulfatés dans des préparations pharmaceutiques, telles que des préparations d’héparines ou des dispositifs médicaux thérapeutiques. Lorsqu’ils sont utilisés pour détecter ou quantifier des glycosaminoglycanes sur-sulfatés dans des solutions contenant de l’héparine, les échantillons sont préparés par un traitement avec des héparinases afin de dégrader l'héparine. Le titrage de l’inhibition de l'activité des polymérases permet la quantification des glycosaminoglycanes sur-sulfatés dans l'échantillon.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PE, PG, PH, PL, PT, RO, RS, RU, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW.
African Regional Intellectual Property Organization (BW, GH, GM, KE, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG).
Publication Language: English (EN)
Filing Language: English (EN)