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1. WO2010027877 - SMALL MOLECULE FLUORESCENT SENSORS FOR DETECTION OF POST-TRANSLATIONAL MODIFICATIONS AND PROTEIN-PROTEIN INTERACTIONS IN BIOASSAYS

Publication Number WO/2010/027877
Publication Date 11.03.2010
International Application No. PCT/US2009/055097
International Filing Date 26.08.2009
IPC
G01N 33/52 2006.1
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
52Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper
G01N 33/68 2006.1
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
68involving proteins, peptides or amino acids
CPC
C12Q 1/37
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
34involving hydrolase
37involving peptidase or proteinase
C12Q 1/42
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
34involving hydrolase
42involving phosphatase
C12Q 1/44
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
34involving hydrolase
44involving esterase
C12Q 1/485
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
48involving transferase
485involving kinase
G01N 33/542
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
536with immune complex formed in liquid phase
542with steric inhibition or signal modification, e.g. fluorescent quenching
G01N 33/6803
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
68involving proteins, peptides or amino acids
6803General methods of protein analysis not limited to specific proteins or families of proteins
Applicants
  • GYRASOL TECHNOLOGIES, INC. [US]/[US] (AllExceptUS)
  • RININSLAND, Frauke, H. [US]/[US] (UsOnly)
  • WITTENBURG, Shannon [US]/[US] (UsOnly)
  • WEATHERFORD, Wendy, L. [US]/[US] (UsOnly)
Inventors
  • RININSLAND, Frauke, H.
  • WITTENBURG, Shannon
  • WEATHERFORD, Wendy, L.
Agents
  • CARROLL, Lawrence, J.
Priority Data
61/146,05321.01.2009US
61/190,12926.08.2008US
Publication Language English (en)
Filing Language English (EN)
Designated States
Title
(EN) SMALL MOLECULE FLUORESCENT SENSORS FOR DETECTION OF POST-TRANSLATIONAL MODIFICATIONS AND PROTEIN-PROTEIN INTERACTIONS IN BIOASSAYS
(FR) DÉTECTEURS FLUORESCENTS À PETITES MOLÉCULES POUR DÉTECTER DES MODIFICATIONS POST-TRADUCTIONNELLES ET DES INTERACTIONS PROTÉINE-PROTÉINE DANS DES BIODOSAGES
Abstract
(EN) The present invention relates to novel compounds which are capable as acting as fluorescent sensors or which are precursors for these and for the use of these for the assay of biological processes such as posttranslational modifications of biological molecules such as phosphorylation, de-phosphorylation, proteolytic cleavage, phosphodiesterase mediated hydrolysis of cyclic nucleotides, methylation, acetylation of proteins peptides, DNA, lipids and the detection of biomolecule interactions (e.g., protein-protein interactions). A small molecule sensor is described which can associate to phosphorylated biological targets via metal ion - phosphate association. The association event can be monitored as fluorescence quench, sensitized emission, fluorescence polarization or a combination thereof. The sensor is useful for determining enzyme activities in a homogeneous assay format and/or in a multiplex format.
(FR) La présente invention porte sur de nouveaux composés qui sont susceptibles de servir de détecteurs fluorescents ou qui sont des précurseurs de ceux-ci, et sur l'utilisation de ceux-ci pour le dosage de processus biologiques tels que des modifications post-traductionnelles de molécules biologiques telles que la phosphorylation, la déphosphorylation, le clivage protéolytique, l'hydrolyse médiée par phosphodiestérase de nucléotides cycliques, la méthylation, l'acétylation de protéines, peptides, ADN, lipides, et la détection d'interactions de biomolécules (par exemple, les interactions protéine-protéine). L'invention porte sur un détecteur à petites molécules qui peut s'associer à des cibles biologiques phosphorylées par le biais d'une association ion métallique–phosphate. L'événement d'association peut être surveillé sous la forme d'une désactivation de fluorescence, d'une émission sensibilisée, d'une polarisation de fluorescence ou d'une combinaison de celles-ci. Le détecteur est utile pour déterminer des activités enzymatiques dans un format de dosage homogène et/ou dans un format multiplex.
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