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1. (WO2010025404) HIGH-THROUGHPUT AND NON-INVASIVE METHOD TO VITRIFY PORCINE EMBRYOS
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/2010/025404    International Application No.:    PCT/US2009/055424
Publication Date: 04.03.2010 International Filing Date: 28.08.2009
IPC:
C12N 5/02 (2006.01), A61D 19/04 (2006.01), A01K 67/00 (2006.01)
Applicants: THE CURATORS OF THE UNIVERSITY OF MISSOURI [US/US]; 316 University Hall Columbia, Missouri 65211 (US) (For All Designated States Except US).
PRATHER, Randall, S. [US/US]; (US) (For US Only).
LI, Rongfeng [CN/US]; (US) (For US Only).
MURPHY, Clifton, N. [US/US]; (US) (For US Only).
SPATE, Lee [US/US]; (US) (For US Only)
Inventors: PRATHER, Randall, S.; (US).
LI, Rongfeng; (US).
MURPHY, Clifton, N.; (US).
SPATE, Lee; (US)
Agent: MCBRIDE, Timothy, B.; (US)
Priority Data:
61/190,515 29.08.2008 US
Title (EN) HIGH-THROUGHPUT AND NON-INVASIVE METHOD TO VITRIFY PORCINE EMBRYOS
(FR) PROCÉDÉ À HAUT RENDEMENT ET NON VULNÉRANT DE VITRIFICATION D’EMBRYONS DE PORC
Abstract: front page image
(EN)The present invention provides a practical, non-invasive, and efficient method for cryopreservation of an in-vitro-produced porcine embryo. The inventive method treats the IVP (such as IVF- or NT-derived) embryo at the one-cell or cleavage stage prior to compaction with high osmolality followed by high speed centrifugation. The high osmolality treatment enlarges the periviteline space, and with centrifugation, enables the separation of the lipids from the cytoplasm. The lipid-separated embryos after high osmolality treatment have been successfully cryopreserved and later recovered and transferred to produce live offspring.
(FR)L’invention concerne un procédé pratique, non vulnérant et efficace de cryoconservation d’un embryon de porc produit in vitro (PPV). Le procédé consiste à soumettre l’embryon PPV (p. ex. dérivé d'une FIV ou d'un transfert de noyau) au stade unicellulaire ou au stade de clivage avant le compactage à un traitement à osmolalité élevée, puis à une centrifugation à grande vitesse. Le traitement à osmolalité élevée agrandit l’espace périvitellin, et, grâce à la centrifugation, permet de séparer les lipides du cytoplasme. Les embryons aux lipides séparés, après le traitement à osmolalité élevée, ont pu être cryoconservés avec succès et récupérés ultérieurement pour produire une descendance vivante.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PE, PG, PH, PL, PT, RO, RS, RU, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW.
African Regional Intellectual Property Organization (BW, GH, GM, KE, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG).
Publication Language: English (EN)
Filing Language: English (EN)