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1. (WO2010024609) NANOPARTICLE SENSOR FOR MEASURING PROTEASE ACTIVITY AND METHOD FOR MANUFACTURING THE SAME
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/2010/024609    International Application No.:    PCT/KR2009/004794
Publication Date: 04.03.2010 International Filing Date: 27.08.2009
IPC:
C12Q 1/37 (2006.01)
Applicants: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY [KR/KR]; 39-1 Hawolgok-Dong, Seongbuk-Gu Seoul 136-791 (KR) (For All Designated States Except US).
THE ASAN FOUNDATION [KR/KR]; 388-1 Pungnap 2-Dong, Songpa-Gu Seoul 138-878 (KR) (For All Designated States Except US).
FUTURECHEM CO., LTD. [KR/KR]; Chemistry Department of Inha University 253, Yonghyeon-Dong, Nam-Gu Incheon 402-020 (KR) (For All Designated States Except US).
KWON, Ick-Chan [KR/KR]; (KR) (For US Only).
CHOI, Kui-Won [KR/KR]; (KR) (For US Only).
KIM, Kwang-Meyung [KR/KR]; (KR) (For US Only).
YOUN, In-Chan [KR/KR]; (KR) (For US Only).
LEE, Seul-Ki [KR/KR]; (KR) (For US Only).
PARK, Kyeong-Soon [KR/KR]; (KR) (For US Only).
MOON, Dae-Hyuk [KR/KR]; (KR) (For US Only).
CHI, Dae-Yoon [KR/KR]; (KR) (For US Only).
LEE, Seung-Jin [KR/KR]; (KR) (For US Only).
MYUNG, Seung-Jae [KR/KR]; (KR) (For US Only)
Inventors: KWON, Ick-Chan; (KR).
CHOI, Kui-Won; (KR).
KIM, Kwang-Meyung; (KR).
YOUN, In-Chan; (KR).
LEE, Seul-Ki; (KR).
PARK, Kyeong-Soon; (KR).
MOON, Dae-Hyuk; (KR).
CHI, Dae-Yoon; (KR).
LEE, Seung-Jin; (KR).
MYUNG, Seung-Jae; (KR)
Agent: PARK, Jang-Won; 3rd Floor, Shinyoung Wacoal Bldg. 49-4 Nonhyun-dong, Gangnam-gu Seoul 135-814 (KR)
Priority Data:
10-2008-0085497 29.08.2008 KR
Title (EN) NANOPARTICLE SENSOR FOR MEASURING PROTEASE ACTIVITY AND METHOD FOR MANUFACTURING THE SAME
(FR) CAPTEUR DE NANOPARTICULES POUR MESURER L'ACTIVITÉ PROTÉASE ET PROCÉDÉ DE PRODUCTION DE CE DERNIER
(KO) 단백질분해효소 활성 측정용 나노입자 센서 및 그 제조방법
Abstract: front page image
(EN)The present invention relates to a nanoparticle sensor for measuring protease activity for protease imaging and a method for manufacturing the same. More specifically, the present invention relates to a nanoparticle sensor for measuring protease activity in which a fluorophore- and quencher-conjugated peptide substrate is conjugated to a biocompatible polymer nanoparticle. The peptide substrate is specifically lysed by a protease. The sensor according to the present invention is capable of inhibiting emission of fluorescence with high extinctive activity of the quencher on a fluorescent material. But strong fluorescence is specifically emitted only if the peptide substrate is lysed by a specific protease. Therefore, the sensor is especially useful as a method for screening a novel drug such as a protease overexpression inhibitor, and early diagnosis of incurable diseases and various diseases such as autoimmune diseases including cancer, osteoarthritis, rheumatoid arthritis, and dementia.
(FR)La présente invention concerne un capteur de nanoparticules pour mesurer l'activité protéase dans le cas de l'imagerie avec des protéases et un procédé de production correspondant. Plus spécifiquement, la présente invention porte sur un capteur de nanoparticules utilisé pour mesurer l'activité protéase dans lequel un substrat peptidique conjugué à un fluorophore et à un extincteur de luminescence est conjugué à une nanoparticule de polymère biocompatible. Le substrat peptidique est spécifiquement lysé par une protéase. Le capteur selon la présente invention peut inhiber l'émission de fluorescence avec une forte activité d'extinction de l'extincteur de luminescence placé sur une matière fluorescente. Cependant, une forte fluorescence est spécifiquement émise uniquement si le substrat peptidique est lysé par une protéase spécifique. Par conséquent, le capteur est spécifiquement utile en tant que procédé de criblage d'un nouveau médicament tel qu'un inhibiteur de surexpression de protéase et pour le diagnostic précoce de maladies incurables et de diverses maladies telles que les maladies autoimmunes incluant le cancer, l'arthrose, la polyarthrite rhumatoïde et la démence.
(KO)본 발명은 단백질분해효소의 영상화를 위한, 단백질분해효소 활성 측정용 나노입자 센서 및 그 제조방법에 관한 것이다. 보다 상세하게는, 형광체 및 소광체가 결합된, 단백질분해효소에 의해 특이적으로 분해되는 펩타이드 기질이 생체 적합성 고분자 나노입자에 결합된 단백질분해효소 활성 측정용 나노입자 센서에 관한 것이다. 본 발명에 따른 센서는 소광체의 형광물질에 대한 높은 소광 능력으로 형광이 발광하지 않은 상태로 있다가 특정 단백질분해효소에 의하여 펩타이드 기질이 분해가 되어야만 강한 형광을 특이적으로 발광함으로써, 특히, 단백질분해효소 과다 발현 억제제와 같은 신약을 스크리닝하는 방법, 암, 골 관절염, 류마티스 관절염, 치매 등 자가면역질환과 같은 다양한 질병 및 난치병의 조기 진단에 유용하게 사용될 수 있다.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PE, PG, PH, PL, PT, RO, RS, RU, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW.
African Regional Intellectual Property Organization (BW, GH, GM, KE, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, SE, SI, SK, SM, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG).
Publication Language: Korean (KO)
Filing Language: Korean (KO)