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1. WO2010003493 - RAPID ANALYTICAL METHOD FOR MIXED BIOLOGICAL SAMPLES

Publication Number WO/2010/003493
Publication Date 14.01.2010
International Application No. PCT/EP2009/004078
International Filing Date 06.06.2009
IPC
C12Q 1/68 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
C12N 15/10 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
10Processes for the isolation, preparation or purification of DNA or RNA
CPC
C12Q 1/6806
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
Applicants
  • QIAGEN GMBH [DE]/[DE] (AllExceptUS)
  • HIMMELREICH, Ralf [DE]/[DE] (UsOnly)
  • HOLZER, Eva [DE]/[DE] (UsOnly)
Inventors
  • HIMMELREICH, Ralf
  • HOLZER, Eva
Agents
  • ENGELS, Barbara
Priority Data
10 2008 032 501.510.07.2008DE
Publication Language German (DE)
Filing Language German (DE)
Designated States
Title
(DE) SCHNELLES ANALYSEVERFAHREN BIOLOGISCHER MISCHPROBEN
(EN) RAPID ANALYTICAL METHOD FOR MIXED BIOLOGICAL SAMPLES
(FR) PROCÉDÉ D'ANALYSE RAPIDE D'ÉCHANTILLONS BIOLOGIQUES MIXTES
Abstract
(DE)
Die vorliegende Erfindung betrifft ein schnelles Verfahren zur Amplifikation von Nukleinsäuren aus biologischen Proben, die ein Gemisch verschiedener Zellen oder ein Gemisch von Zellen mit verunreinigenden Komponenten enthalten, bei dem solche Zellen in einer Lyselösung (A) lysiert werden und das Lysat unmittelbar anschließend für die Analyse mithilfe eines Nukleinsäure amplifizierenden Verfahrens eingesetzt werden kann.
(EN)
The present invention relates to a rapid method for the amplification of nucleic acids from biological samples which contain a mixture of different cells or a mixture of cells with contaminating components, in which such cells are lysed in a lysis solution (A) and the lysate can be used immediately subsequently for the analysis using a method amplifying nucleic acid.
(FR)
La présente invention porte sur un procédé rapide pour l'amplification d'acides nucléiques provenant d'échantillons biologiques, qui contiennent un mélange de différentes cellules ou un mélange de cellules avec des composants contaminants. Selon ce procédé, de telles cellules sont lysées dans une solution de lyse (A) et le lysat peut être utilisé immédiatement après pour l'analyse à l'aide d'un procédé d'amplification d'acide nucléique.
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