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1. WO2010002552 - PROCESS FOR CONCENTRATING AND PROCESSING FLUID SAMPLES

Publication Number WO/2010/002552
Publication Date 07.01.2010
International Application No. PCT/US2009/046755
International Filing Date 09.06.2009
IPC
B01D 61/00 2006.01
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
DSEPARATION
61Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
C12Q 1/04 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
02involving viable microorganisms
04Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
C12Q 1/24 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
02involving viable microorganisms
24Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganism
G01N 33/487 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
483Physical analysis of biological material
487of liquid biological material
CPC
C12Q 1/24
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
02involving viable microorganisms
24Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms
Applicants
  • GENERAL ELECTRIC COMPANY [US]/[US] (AllExceptUS)
  • BOYETTE, Scott [US]/[US] (UsOnly)
  • XU, Rong [CN]/[CN] (UsOnly)
  • LUO, Jing [CN]/[CN] (UsOnly)
  • XU, Weiqing [CN]/[CN] (UsOnly)
  • CHEN, Jing [CN]/[CN] (UsOnly)
  • XIAO, Weimin [CN]/[CN] (UsOnly)
  • CHEN, Tong [US]/[CN] (UsOnly)
Inventors
  • BOYETTE, Scott
  • XU, Rong
  • LUO, Jing
  • XU, Weiqing
  • CHEN, Jing
  • XIAO, Weimin
  • CHEN, Tong
Agents
  • GNIBUS, Michael, M.
Priority Data
12/215,76830.06.2008US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) PROCESS FOR CONCENTRATING AND PROCESSING FLUID SAMPLES
(FR) PROCÉDÉ DE CONCENTRATION ET DE TRAITEMENT D'ÉCHANTILLONS DE FLUIDE
Abstract
(EN)
A method of treating a liquid sample having microbiological target species therein to concentrate the species and collect lysate is disclosed. The liquid sample comprises non-target microbiological particles, inorganic particles, and microbiological target species. The liquid is passed through a prefilter medium to allow the target species to pass through as filtrate and retain non-target microbiological products and inorganic particles thereon. The filtrate is contacted with a main filtration medium adapted to retain the target species thereon as retentate. The retentate is lysed to form a lysate containing target material that was enveloped within the microbiological target species. The microbiological target species may comprise cellular organisms such as bacteria, algae, fungi, prokaryotes, etc. or viruses. Target materials comprise intracellular nucleic acids, or in the case of viral sampling, nucleic acids encased within the protein sheath or coating of the virus.
(FR)
L'invention concerne un procédé de traitement d'un échantillon liquide contenant des espèces cibles microbiologiques, permettant de concentrer les espèces et de collecter le lysat. L'échantillon liquide contient des particules microbiologiques non cibles, des particules inorganiques et des espèces cibles microbiologiques. Le liquide est passé à travers un milieu de préfiltre qui permet aux espèces cibles de le traverser en tant que filtrat et qui retient les produits microbiologiques non cibles et les particules inorganiques. Le filtrat est mis en contact avec un milieu de filtration principal conçu pour retenir les espèces cibles en tant que rétentat. Ce rétentat est lysé pour former un lysat contenant une matière cible enveloppée dans les espèces cibles microbiologiques. Ces dernières peuvent comprendre des organismes cellulaires tels que des bactéries, des algues, des champignons, des procaryotes, etc. ou des virus. Les matières cibles comprennent des acides nucléiques intracellulaires ou, dans le cas de l'échantillonnage viral, des acides nucléiques enrobés dans la gaine protéique ou l'enveloppe du virus.
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