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1. WO2010001162 - FREEZE-DRIED COMPOSITIONS FOR BIOCHEMICAL REACTIONS

Publication Number WO/2010/001162
Publication Date 07.01.2010
International Application No. PCT/GB2009/050765
International Filing Date 01.07.2009
IPC
C12Q 1/68 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
G01N 33/548 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
543with an insoluble carrier for immobilising immunochemicals
544the carrier being organic
548Carbohydrates, e.g. dextran
CPC
C07H 21/00
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
21Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
C12Q 1/6806
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
Applicants
  • ENIGMA DIAGNOSTICS LTD [GB]/[GB] (AllExceptUS)
  • FRANKS, Felix [GB]/[GB] (UsOnly)
  • SPIERS, Samantha [GB]/[GB] (UsOnly)
  • LEE, Martin [GB]/[GB] (UsOnly)
  • SUTTON, Diane [GB]/[GB] (UsOnly)
Inventors
  • FRANKS, Felix
  • SPIERS, Samantha
  • LEE, Martin
  • SUTTON, Diane
Agents
  • GREAVES, Carol
Priority Data
0812041.202.07.2008GB
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) FREEZE-DRIED COMPOSITIONS FOR BIOCHEMICAL REACTIONS
(FR) COMPOSITIONS LYOPHILISÉES POUR DES RÉACTIONS BIOCHIMIQUES
Abstract
(EN)
The use of raffinose as a glass-forming agent for freeze-dried compositions intended for use in conducting chemical or biochemical reactions such as the PCR. Thus, for instance, there is provided a composition for carrying out a chemical or biochemical reaction, said composition being in a freeze-dried formand comprising (i) a set of reagents comprising at least some of the chemical or biochemical reagents necessaryfor conducting said chemical or biochemical reaction, and (ii) raffinose. Kits comprising these compositions and methods of using them form a further aspect of the invention.
(FR)
L'invention concerne l'utilisation de raffinose en tant qu'agent de formation de verre pour des compositions lyophilisées destinées à être utilisées pour effectuer des réactions chimiques ou biochimiques tels que la PCR. Ainsi, par exemple, l'invention porte sur une composition pour effectuer une réaction chimique ou biochimique, ladite composition étant dans une forme lyophilisée et comprenant (i) un ensemble de réactifs comprenant au moins une partie des réactifs chimiques ou biochimiques nécessaires à la conduite de ladite réaction chimique ou biochimique, et (ii) du raffinose. Des trousses comprenant ces compositions et des procédés pour les utiliser forment un autre aspect de l'invention.
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