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1. WO2009147200 - METHOD FOR THE SUPPLY OF GROWTH COMPONENTS TO CELL CULTURES

Publication Number WO/2009/147200
Publication Date 10.12.2009
International Application No. PCT/EP2009/056861
International Filing Date 04.06.2009
IPC
C12N 1/20 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
20Bacteria; Culture media therefor
CPC
C12N 1/20
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1Microorganisms, e.g. protozoa; Compositions thereof
20Bacteria
C12N 5/0018
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor;
0018Culture media for cell or tissue culture
C12P 21/02
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
21Preparation of peptides or proteins
02having a known sequence of two or more amino acids, e.g. glutathione
Applicants
  • BioSilta Oy [FI]/[FI] (AllExceptUS)
  • NEUBAUER, Peter [DE]/[DE] (UsOnly)
  • VASALA, Antti [FI]/[FI] (UsOnly)
  • GOLSON, Russell [GB]/[GB] (UsOnly)
Inventors
  • NEUBAUER, Peter
  • VASALA, Antti
  • GOLSON, Russell
Agents
  • GANAHL ET AL., Bernhard
Priority Data
08157594.604.06.2008EP
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) METHOD FOR THE SUPPLY OF GROWTH COMPONENTS TO CELL CULTURES
(FR) PROCÉDÉ DE FOURNITURE DE COMPOSANTS DE CROISSANCE À DES CULTURES CELLULAIRES
Abstract
(EN)
The present invention provides a method for improving the preparation and use of growth media by the uses of specific pellet formulations, which especially are tablets of different sizes, which contain the growth medium or parts thereof and are sterilized with the standard methods of pharmaceutical technology. Specifically these pellet formulations are applied to control a cell culture in a way that the adaptation phase is shorter or that the growth is controlled by a release of certain components at a certain time and in a certain concentration during the process, and nutrients (e.g., nitrogen) can be packed into the cultivation vessel in amounts sufficient for high cell densities without the risk of intoxication of the organism.
(FR)
La présente invention porte sur un procédé d'amélioration de la préparation et de l'utilisation de milieux de croissance par les utilisations de formulations de pastilles spécifiques, qui sont notamment des comprimés de différentes dimensions, lesquelles contiennent le milieu de croissance ou des parties de celui-ci et sont stérilisées par les procédés standards de technologie pharmaceutique. De façon spécifique, ces formulations de pastilles sont appliquées pour contrôler une culture cellulaire de telle sorte que la phase d'adaptation est plus courte ou que la croissance est contrôlée par une libération de certains composants à un certain moment et dans une certaine concentration durant le procédé, et des nutriments (par exemple, de l'azote) peuvent être conditionnés dans le récipient de culture dans des quantités suffisantes pour des densités cellulaires élevées sans le risque d'intoxication de l'organisme.
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