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1. (WO2009131948) HIGH-THROUGHPUT CELL-BASED CFTR ASSAY
Latest bibliographic data on file with the International Bureau   

Pub. No.: WO/2009/131948 International Application No.: PCT/US2009/041147
Publication Date: 29.10.2009 International Filing Date: 20.04.2009
IPC:
C12Q 1/68 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
68
involving nucleic acids
Applicants:
GARDENER, Matthew, James [GB/GB]; GB (UsOnly)
SHEARER, Joanne, Lesley [GB/GB]; GB (UsOnly)
CRONK, David, William [GB/GB]; GB (UsOnly)
INSTITUTE FOR ONEWORLD HEALTH [US/US]; 50 California Street, Suite 500 San Francisco, CA 94111, US (AllExceptUS)
Inventors:
GARDENER, Matthew, James; GB
SHEARER, Joanne, Lesley; GB
CRONK, David, William; GB
Agent:
KONSKI, Antoinette, F.; US
Priority Data:
61/046,74621.04.2008US
Title (EN) HIGH-THROUGHPUT CELL-BASED CFTR ASSAY
(FR) DOSAGE À BASE CELLULAIRE ET À HAUT RENDEMENT DE LA CFTR
Abstract:
(EN) This invention provides a high throughput screen for measuring the transport of an ion through a cystic fibrosis transmembrane conductance regulator (CFTR) of a cell that endogenously expresses CFTR. The method requires culturing the cell that endogenously expresses CFTR in the presence of an ion-sensitive compound and then contacting the cell with a CFTR activator. After a suitable amount of time after addition of the activator, the test compound is added to the cell culture medium. Thereafter, any change in the ion-sensitive compound is measured by any suitable method that can detect the change in the ion-sensitive compound, i.e. by measuring a change in the light emitted from a fluorescent compound, which can be recorded by an imaging plate reader.
(FR) La présente invention concerne un criblage à haut débit permettant la mesure du transport d'un ion par un régulateur de la conductance transmembranaire de la mucoviscidose (CFTR) d'une cellule exprimant, de façon endogène, ledit CFTR. Ce procédé implique la culture de la cellule exprimant de façon endogène le CFTR en présence d'un composé sensible aux ions, cela étant suivi de la mise en contact de la cellule avec un activateur du CFTR. A l'issue d'un laps de temps suffisant après l'addition de l'activateur, le composé à étudier est ajouté au milieu de culture de la cellule. Par la suite, toute modification affectant le composé sensible aux ions est mesurée par un quelconque procédé adapté capable de détecter ladite modification affectant le composé sensible aux ions, par exemple grâce à la mesure d'une modification affectant la lumière émise par un composé fluorescent, qui peut être enregistrée par un lecteur de plaques d'imagerie.
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BR, BW, BY, BZ, CA, CH, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PG, PH, PL, PT, RO, RS, RU, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Office (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (EPO) (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, SE, SI, SK, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)