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1. (WO2009122580) METHOD OF BIOASSAYING YOKUKANSAN
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/2009/122580    International Application No.:    PCT/JP2008/056684
Publication Date: 08.10.2009 International Filing Date: 03.04.2008
IPC:
G01N 33/15 (2006.01), A61K 36/18 (2006.01), A61P 25/28 (2006.01), C12N 5/06 (2006.01), G01N 33/50 (2006.01)
Applicants: TSUMURA & Co. [JP/JP]; 2-17-11, Akasaka, Minato-ku, Tokyo, 1078521 (JP) (For All Designated States Except US).
IKARASHI, Yasushi [JP/JP]; (JP) (For US Only).
KAWAKAMI, Zenji [JP/JP]; (JP) (For US Only)
Inventors: IKARASHI, Yasushi; (JP).
KAWAKAMI, Zenji; (JP)
Agent: The Patent Corporate body of Ono & Co.; Mitobe Bldg. 4F, 1-13-1, Kandaizumi-cho, Chiyoda-ku Tokyo 1010024 (JP)
Priority Data:
Title (EN) METHOD OF BIOASSAYING YOKUKANSAN
(FR) PROCÉDÉ D'ANALYSE BIOLOGIQUE DE YOKUKANSAN
(JA) 抑肝散のバイオアッセイ方法
Abstract: front page image
(EN)It is intended to find an in vitro bioassay system capable of ensuring improved qualities yokukansan (a Chinese medicine). Thus, a method of bioassaying yokukansan, which is characterized by comprising adding glutamic acid in an amount sufficient for inducing cell death and yokukansan to a medium in which cells are to be cultured and evaluating the pharmacological activity of yokukansan based on the survival rate of the cells having been cultured in the medium, is provided.
(FR)Un système d'analyse biologique in vitro capable de garantir des qualités améliorées du yokukansan (un médicament chinois) était recherché. Ainsi, l'invention concerne un procédé d'analyse biologique de yokukansan, caractérisé en ce qu'il comprend l'addition d'acide glutamique, en une quantité suffisante pour induire la mort cellulaire, et de yokukansan, à un milieu dans lequel des cellules doivent être cultivées, et l'évaluation de l'activité pharmacologique du yokukansan d'après le taux de survie des cellules qui ont été cultivées dans le milieu.
(JA) 抑肝散について、より高い品質保証を可能とする、試験管内試験によるバイオアッセイ系を見出すことを目的とし、培養細胞を培養する培地中に、細胞死を誘発するのに十分な量のグルタミン酸と、抑肝散を添加し、培地中の培養細胞の生存率から抑肝散の薬理活性価を評価することを特徴とする抑肝散のバイオアッセイ方法を提供する。  
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BR, BW, BY, BZ, CA, CH, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PG, PH, PL, PT, RO, RS, RU, SC, SD, SE, SG, SK, SL, SM, SV, SY, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW.
African Regional Intellectual Property Organization (BW, GH, GM, KE, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MT, NL, NO, PL, PT, RO, SE, SI, SK, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG).
Publication Language: Japanese (JA)
Filing Language: Japanese (JA)