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1. (WO2009116863) EXPRESSION-LINKED GENE DISCOVERY
Latest bibliographic data on file with the International Bureau

Pub. No.: WO/2009/116863 International Application No.: PCT/NL2009/050128
Publication Date: 24.09.2009 International Filing Date: 17.03.2009
IPC:
C12Q 1/68 (2006.01) ,C12N 15/10 (2006.01)
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Q
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1
Measuring or testing processes involving enzymes or micro-organisms; Compositions therefor; Processes of preparing such compositions
68
involving nucleic acids
C CHEMISTRY; METALLURGY
12
BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N
MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15
Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09
Recombinant DNA-technology
10
Processes for the isolation, preparation or purification of DNA or RNA
Applicants:
EXPRESSIVE RESEARCH B.V. [NL/NL]; Nieuwe Kanaal 7b NL-6709 PA Wageningen, NL (AllExceptUS)
DE BOER, Anne Douwe [NL/NL]; NL (UsOnly)
EBSKAMP, Michaël Johannes Marcus [NL/NL]; NL (UsOnly)
LANGEVELD, Simon Albertus [NL/NL]; NL (UsOnly)
LAROS, Ivo [NL/NL]; NL (UsOnly)
VAN DE RHEE, Miranda, Debora [NL/NL]; NL (UsOnly)
Inventors:
DE BOER, Anne Douwe; NL
EBSKAMP, Michaël Johannes Marcus; NL
LANGEVELD, Simon Albertus; NL
LAROS, Ivo; NL
VAN DE RHEE, Miranda, Debora; NL
Agent:
HATZMANN, M., J.; Vereenigde Johan de Wittlaan 7 NL-2517 JR Den Haag, NL
Priority Data:
08152859.817.03.2008EP
Title (EN) EXPRESSION-LINKED GENE DISCOVERY
(FR) DÉCOUVERTE DE GÈNES LIÉS À L'EXPRESSION
Abstract:
(EN) The invention relates to a method for analysing a genomicregion of an organism, comprising four major parts. The first part involves the isolation of mRNA from a selected organism that is used for the preparation of small single stranded DNA fragments with one adaptor containing an affinity label. These DNA fragments are used in part three. In the second part genomic DNA from the same or a related organism is isolated. This genomic DNA is fragmented and ligated to adaptor molecules. In the third part these genomicfragments are hybridized with single stranded DNA fragments from part one and the hybrids formed inthis process are used for synthesis of DNA fragments. These fragments will be used in part four, which involves sequencing of these fragments using one of the available high throughput sequencing methods.
(FR) L'invention concerne un procédé d'analyse d'une microrégion génomique d'un organisme, comportant quatre parties principales. La première partie implique l'isolement d'ARNm à partir d'un organisme sélectionné utilisé pour la préparation de petits fragments d'ADN à simple brin avec un adaptateur contenant un marqueur d'affinité. Ces fragments d'ADN sont utilisés dans la troisième partie. Dans la deuxième partie, on isole l'ADN génomique du même organisme ou d'un organisme apparenté. On fragmente cet ADN génomique et on le ligature à des molécules adaptatrices. Dans la troisième partie, on hybride ces fragments génomiques avec des fragments d'ADN à simple brin de la première partie et on utilise les hybrides formés dans ce processus pour la synthèse des fragments d'ADN. Ces fragments seront utilisés dans la quatrième partie qui implique le séquençage de ces fragments à l'aide de l'une des méthodes de séquençage à haut débit disponibles.
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Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BR, BW, BY, BZ, CA, CH, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PG, PH, PL, PT, RO, RS, RU, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW
African Regional Intellectual Property Organization (ARIPO) (BW, GH, GM, KE, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, SE, SI, SK, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG)
Publication Language: English (EN)
Filing Language: English (EN)
Also published as:
AR070929IL208237EP2268834US20110105338RU2010142289CN102027136
CA2718905ES2528971BRPI0908734NZ588112DK2268834AU2009226248
IN6457/CHENP/2010