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1. (WO2009057813) METHOD FOR HIGH-LEVEL SECRETION AND PRODUCTION OF PROTEIN
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/2009/057813    International Application No.:    PCT/JP2008/070155
Publication Date: 07.05.2009 International Filing Date: 29.10.2008
IPC:
C12N 15/00 (2006.01), C12N 5/00 (2006.01)
Applicants: NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY [JP/JP]; 3-1, Kasumigaseki 1-chome, Chiyoda-ku, Tokyo 1008921 (JP) (JP only).
Kyowa Hakko Kirin Co., Ltd. [JP/JP]; 1-6-1, Ohtemachi, Chiyoda-ku, Tokyo 1008185 (JP) (For All Designated States Except US).
DAIICHI SANKYO COMPANY, LIMITED [JP/JP]; 3-5-1, Nihonbashi Honcho, Chuo-ku, Tokyo 1038426 (JP) (For All Designated States Except US).
CHIBA, Yasunori [JP/JP]; (JP) (For US Only).
JIGAMI, Yoshifumi [JP/JP]; (JP) (For US Only).
TAKAHASHI, Yoshie [JP/JP]; (JP) (For US Only).
KURODA, Kosuke [JP/JP]; (JP) (For US Only).
KOBAYASHI, Kazuo [JP/JP]; (JP) (For US Only).
ICHIKAWA, Kimihisa [JP/JP]; (JP) (For US Only).
NONAKA, Koichi [JP/JP]; (JP) (For US Only).
SUZUKI, Takeshi [JP/JP]; (JP) (For US Only).
ONO, Minako [JP/JP]; (JP) (For US Only)
Inventors: CHIBA, Yasunori; (JP).
JIGAMI, Yoshifumi; (JP).
TAKAHASHI, Yoshie; (JP).
KURODA, Kosuke; (JP).
KOBAYASHI, Kazuo; (JP).
ICHIKAWA, Kimihisa; (JP).
NONAKA, Koichi; (JP).
SUZUKI, Takeshi; (JP).
ONO, Minako; (JP)
Agent: HIRAKI, Yusuke; Kamiya-cho MT Bldg. 19F, 3-20, Toranomon 4-chome, Minato-ku, Tokyo 1050001 (JP)
Priority Data:
2007-283731 31.10.2007 JP
2007-288845 06.11.2007 JP
Title (EN) METHOD FOR HIGH-LEVEL SECRETION AND PRODUCTION OF PROTEIN
(FR) PROCÉDÉ DE SÉCRÉTION ET DE PRODUCTION À UN NIVEAU ÉLEVÉ D'UNE PROTÉINE
(JA) タンパク質の高分泌生産方法
Abstract: front page image
(EN)Disclosed is a means for secreting and producing a protein, particularly a protein having a complicated structure (e.g., an antibody), in a host cell (e.g., an yeast cell) at a high level. Specifically disclosed are: a transformed host cell having one or more chaperone protein genes; and a method for secreting and producing a foreign protein at a high level by suppressing an O-type sugar chain which is specific to a host cell such as an yeast cell by using the transformed host cell.
(FR)L'invention porte sur un moyen pour secréter et produire une protéine, en particulier une protéine ayant une structure compliquée (par exemple un anticorps) dans une cellule hôte (par exemple une cellule de levure) à un niveau élevé. De façon spécifique, l'invention porte sur une cellule hôte transformée ayant un ou plusieurs gènes de protéine chaperon et sur un procédé de sécrétion et de production d'une protéine étrangère à un niveau élevé par suppression d'une chaîne de sucre de type O qui est spécifique à une cellule hôte telle qu'une cellule de levure par l'utilisation de la cellule hôte transformée.
(JA)本発明は、酵母などの宿主細胞において、タンパク質、特には抗体などの構造が複雑なタンパク質を高分泌生産する手段を提供することを課題とする。 本発明によれば、1種または2種以上のシャペロンタンパク遺伝子を有する形質転換宿主細胞、該形質転換宿主細胞を用い、かつ酵母などの宿主細胞に特有のO型糖鎖を抑制することによって外来タンパク質を高分泌生産する方法が提供される。
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BR, BW, BY, BZ, CA, CH, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PG, PH, PL, PT, RO, RS, RU, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW.
African Regional Intellectual Property Organization (BW, GH, GM, KE, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MT, NL, NO, PL, PT, RO, SE, SI, SK, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG).
Publication Language: Japanese (JA)
Filing Language: Japanese (JA)