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Machine translation
1. (WO2009056831) POLYMERASE-BASED SINGLE-MOLECULE SEQUENCING
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/2009/056831    International Application No.:    PCT/GB2008/003669
Publication Date: 07.05.2009 International Filing Date: 30.10.2008
IPC:
C12Q 1/68 (2006.01)
Applicants: ISIS INNOVATION LIMITED [GB/GB]; University of Oxford Ewert House Ewert Place Summertown, Oxford 0X2 7SG (GB) (For All Designated States Except US).
KAPANIDIS, Achillefs [GR/GB]; (GB) (For US Only)
Inventors: KAPANIDIS, Achillefs; (GB)
Agent: O'NEILL, Michelle; Harrison IP Limited, Marlborough House Westminster Place York Business Park Nether Poppleton York YO26 6RW (GB)
Priority Data:
0721340.8 30.10.2007 GB
Title (EN) POLYMERASE-BASED SINGLE-MOLECULE SEQUENCING
(FR) SÉQUENÇAGE DE MOLÉCULES INDIVIDUELLES AU MOYEN DE POLYMÉRASES
Abstract: front page image
(EN)The invention relates to an assay method for determining the base sequence in a nucleic acid sample which comprises detection of a single base in said sequence using a polymerase modified to include at least one fluorophore and a dark quencher group; detecting and deducing the amount of energy transfer.
(FR)L'invention concerne un procédé de dosage qui consiste à déterminer de la séquence de bases d'un échantillon d'acide nucléique en détectant une base individuelle dans ladite séquence à l'aide d'une polymérase modifiée comprenant au moins un fluorophore et un groupe quencher non fluorescent (dark quencher - DDQ); à détecter et à déduire la quantité de transfert d'énergie.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BR, BW, BY, BZ, CA, CH, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PG, PH, PL, PT, RO, RS, RU, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW.
African Regional Intellectual Property Organization (BW, GH, GM, KE, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MT, NL, NO, PL, PT, RO, SE, SI, SK, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG).
Publication Language: English (EN)
Filing Language: English (EN)