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1. (WO2009056344) CORE-SHELL MACROMOLECULES FOR SPECIFIC CELL NUCLEUS OR/AND CELL MATRIX STAINING
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/2009/056344    International Application No.:    PCT/EP2008/009224
Publication Date: 07.05.2009 International Filing Date: 31.10.2008
IPC:
C08F 290/00 (2006.01), C08F 285/00 (2006.01), C08F 277/00 (2006.01), C08F 257/00 (2006.01), C08F 289/00 (2006.01)
Applicants: MAX-PLANCK-GESELLSCHAFT ZUR FÖRDERUNG DER WISSENSCHAFTEN E.V. [DE/DE]; Hofgartenstrasse 8, 80539 München (DE) (For All Designated States Except US).
JOHANNES GUTENBERG UNIVERSITÄT MAINZ [DE/DE]; 55122 Mainz (DE) (For All Designated States Except US).
YIN, Meizen [CN/DE]; (DE) (For US Only).
MÜLLEN, Klaus [DE/DE]; (DE) (For US Only).
WEIL, Tanja [DE/DE]; (DE) (For US Only).
SHEN, Jie [CN/DE]; (DE) (For US Only).
PFLUGFELDER, Gert, O. [DE/DE]; (DE) (For US Only)
Inventors: YIN, Meizen; (DE).
MÜLLEN, Klaus; (DE).
WEIL, Tanja; (DE).
SHEN, Jie; (DE).
PFLUGFELDER, Gert, O.; (DE)
Agent: WEICKMANN, F., A.; Weickmann & Weickmann, Postfach 860 820, 81635 München (DE)
Priority Data:
07021426.7 02.11.2007 EP
Title (EN) CORE-SHELL MACROMOLECULES FOR SPECIFIC CELL NUCLEUS OR/AND CELL MATRIX STAINING
(FR) MACROMOLÉCULES NOYAU-ENVELOPPE POUR UNE COLORATION SPÉCIFIQUE DU NOYAU DE CELLULES ET/OU DE LA MATRICE DE CELLULES
Abstract: front page image
(EN)Water-soluble fluorescent core-shell macromolecules containing multiple carboxylic acid groups were synthesized via ATRP by applying perylenediimide derivatives as template. These compounds show high fluorescence quantum yields and photostability in water. Histochemistry and dot blotting experiments show that these macromolecules specifically and rapidly stain the cell nucleus by interaction with histones. Due to their spectral properties, these compounds offer an attractive alternative to existing fluorescent staining methods.
(FR)Selon l'invention, des macromolécules noyau-enveloppe fluorescentes solubles dans l'eau contenant de multiples groupes acides carboxyliques ont été synthétisées par ATRP par application de dérivés de pérylènediimide comme matrice. Ces composés présentent des rendements quantiques de fluorescence élevés et une photostabilité élevée dans l'eau. Des expériences d'histochimie et de transfert en point montrent que ces macromolécules colorent de façon spécifique et rapide le noyau de cellules par interaction avec les histones. En raison de leurs propriétés spectrales, ces composés offrent une alternative attrayante aux procédés de coloration fluorescente existants.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BR, BW, BY, BZ, CA, CH, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PG, PH, PL, PT, RO, RS, RU, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW.
African Regional Intellectual Property Organization (BW, GH, GM, KE, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MT, NL, NO, PL, PT, RO, SE, SI, SK, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG).
Publication Language: English (EN)
Filing Language: English (EN)