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1. WO2009042671 - THREE-DIMENSIONAL MICROFABRICATED BIOREACTORS WITH EMBEDDED CAPILLARY NETWORK

Publication Number WO/2009/042671
Publication Date 02.04.2009
International Application No. PCT/US2008/077503
International Filing Date 24.09.2008
IPC
C12M 1/14 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
1Apparatus for enzymology or microbiology
14with means providing thin layers or with multi-level trays
CPC
B01L 3/502707
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
3Containers or dishes for laboratory use, e.g. laboratory glassware
50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
502with fluid transport, e.g. in multi-compartment structures
5027by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
502707characterised by the manufacture of the container or its components
B33Y 10/00
BPERFORMING OPERATIONS; TRANSPORTING
33ADDITIVE MANUFACTURING TECHNOLOGY
YADDITIVE MANUFACTURING, i.e. MANUFACTURING OF THREE-DIMENSIONAL [3-D] OBJECTS BY ADDITIVE DEPOSITION, ADDITIVE AGGLOMERATION OR ADDITIVE LAYERING, e.g. BY 3-D PRINTING, STEREOLITHOGRAPHY OR SELECTIVE LASER SINTERING
10Processes of additive manufacturing
B81B 2201/058
BPERFORMING OPERATIONS; TRANSPORTING
81MICROSTRUCTURAL TECHNOLOGY
BMICROSTRUCTURAL DEVICES OR SYSTEMS, e.g. MICROMECHANICAL DEVICES
2201Specific applications of microelectromechanical systems
05Microfluidics
058Microfluidics not provided for in B81B2201/051 - B81B2201/054
B81B 2201/06
BPERFORMING OPERATIONS; TRANSPORTING
81MICROSTRUCTURAL TECHNOLOGY
BMICROSTRUCTURAL DEVICES OR SYSTEMS, e.g. MICROMECHANICAL DEVICES
2201Specific applications of microelectromechanical systems
06Bio-MEMS
B81C 1/00119
BPERFORMING OPERATIONS; TRANSPORTING
81MICROSTRUCTURAL TECHNOLOGY
CPROCESSES OR APPARATUS SPECIALLY ADAPTED FOR THE MANUFACTURE OR TREATMENT OF MICROSTRUCTURAL DEVICES OR SYSTEMS
1Manufacture or treatment of devices or systems in or on a substrate
00015for manufacturing microsystems
00023without movable or flexible elements
00119Arrangement of basic structures like cavities or channels, e.g. suitable for microfluidic systems
B81C 99/0095
BPERFORMING OPERATIONS; TRANSPORTING
81MICROSTRUCTURAL TECHNOLOGY
CPROCESSES OR APPARATUS SPECIALLY ADAPTED FOR THE MANUFACTURE OR TREATMENT OF MICROSTRUCTURAL DEVICES OR SYSTEMS
99Subject matter not provided for in other groups of this subclass
0075Manufacture of substrate-free structures
0095Aspects relating to the manufacture of substrate-free structures, not covered by groups B81C99/008 - B81C99/009
Applicants
  • THE BOARD OF TRUSTEES OF THE UNIVERSITY OF ILLINOIS [US]/[US] (AllExceptUS)
  • FANG, Nicholas X. [CN]/[US] (UsOnly)
  • XIA, Chunguang [CN]/[US] (UsOnly)
  • COX, Andrew [US]/[US] (UsOnly)
Inventors
  • FANG, Nicholas X.
  • XIA, Chunguang
  • COX, Andrew
Agents
  • CHAPMAN, Gary B.
Priority Data
60/974,69924.09.2007US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) THREE-DIMENSIONAL MICROFABRICATED BIOREACTORS WITH EMBEDDED CAPILLARY NETWORK
(FR) BIORÉACTEURS TRIDIMENSIONNELS MICROFABRIQUÉS À RÉSEAU CAPILLAIRE INCORPORÉ
Abstract
(EN)
In an aspect, the present invention uses projection micro stereolithography to generate three-dimensional microvessel networks that are capable of supporting and fostering growth of a cell population. For example, provided is a method of making a microvascularized bioreactor via layer-by-layer polymerization of a photocurable liquid composition with repeated patterns of illumination, wherein each layer corresponds to a layer of the desired microvessel network. The plurality of layers are assembled to make a microvascular network. Support structures having different etch rates than the structures that make up the network provides access to manufacturing arbitrary geometries that cannot be made by conventional methods. A cell population is introduced to the external wall of the network to obtain a microvascularized bioreactor. Provided are various methods and related bioreactors, wherein the network wall has a permeability to a biological material that varies within and along the network.
(FR)
Dans un aspect, la présente invention utilise la microstéréolithographie par projection pour générer des réseaux de microvaisseaux tridimensionnels qui sont capables de supporter ou de favoriser la croissance d'une population cellulaire. Par exemple, l'invention concerne un procédé de fabrication d'un bioréacteur microvascularisé par le biais d'une polymérisation couche par couche d'une composition liquide photodurcissable avec des motifs répétés d'éclairage, chaque couche correspondant à une couche souhaitée du réseau de microvaisseaux. La pluralité de couches est assemblée pour construire un réseau microvasculaire. Les structures de support ayant des vitesses de gravure différentes des structures qui constituent le réseau donnent accès à la fabrication de géométries arbitraires qui ne peuvent pas être fabriquées par des procédés classiques. Une population cellulaire est introduite sur la paroi externe du réseau afin d'obtenir un bioréacteur microvascularisé. L'invention concerne également des procédés variés et des bioréacteurs s'y rapportant, la paroi du réseau ayant une perméabilité à un matériau biologique qui varie à l'intérieur et tout au long du réseau.
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