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1. WO2009041577 - URINE PRETREATMENT AGENT FOR URINARY PROTEIN DETERMINATION, URINE PRETREATMENT METHOD, AND URINARY PROTEIN DETERMINATION METHOD

Publication Number WO/2009/041577
Publication Date 02.04.2009
International Application No. PCT/JP2008/067422
International Filing Date 26.09.2008
IPC
G01N 33/48 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
G01N 33/493 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
483Physical analysis of biological material
487of liquid biological material
493urine
G01N 33/543 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
543with an insoluble carrier for immobilising immunochemicals
CPC
G01N 33/6827
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
68involving proteins, peptides or amino acids
6803General methods of protein analysis not limited to specific proteins or families of proteins
6827Total protein determination, e.g. albumin in urine
Y10T 436/10
YSECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
10TECHNICAL SUBJECTS COVERED BY FORMER USPC
TTECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
436Chemistry: analytical and immunological testing
10Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
Y10T 436/107497
YSECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
10TECHNICAL SUBJECTS COVERED BY FORMER USPC
TTECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
436Chemistry: analytical and immunological testing
10Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
107497Preparation composition [e.g., lysing or precipitation, etc.]
Y10T 436/108331
YSECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
10TECHNICAL SUBJECTS COVERED BY FORMER USPC
TTECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
436Chemistry: analytical and immunological testing
10Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
108331Preservative, buffer, anticoagulant or diluent
Applicants
  • 国立大学法人新潟大学 NIIGATA UNIVERSITY [JP]/[JP] (AllExceptUS)
  • デンカ生研株式会社 Denka Seiken Co. ltd. [JP]/[JP] (AllExceptUS)
  • 原 正則 HARA, Masanori [JP]/[JP]
  • 斎藤 亮彦 SAITO, Akihiko [JP]/[JP] (UsOnly)
  • 小笠原 真也 OGASAWARA, Shinya [JP]/[JP] (UsOnly)
  • 平山 吉朗 HIRAYAMA, Yoshiaki [JP]/[JP] (UsOnly)
  • 黒澤 寛之 KUROSAWA, Hiroyuki [JP]/[JP] (UsOnly)
Inventors
  • 原 正則 HARA, Masanori
  • 斎藤 亮彦 SAITO, Akihiko
  • 小笠原 真也 OGASAWARA, Shinya
  • 平山 吉朗 HIRAYAMA, Yoshiaki
  • 黒澤 寛之 KUROSAWA, Hiroyuki
Agents
  • 牛木 護 USHIKI, Mamoru
Priority Data
2007-25170727.09.2007JP
Publication Language Japanese (JA)
Filing Language Japanese (JA)
Designated States
Title
(EN) URINE PRETREATMENT AGENT FOR URINARY PROTEIN DETERMINATION, URINE PRETREATMENT METHOD, AND URINARY PROTEIN DETERMINATION METHOD
(FR) AGENT DE PRÉTRAITEMENT D'URINE POUR LA DÉTERMINATION DE PROTÉINE URINAIRE, PROCÉDÉ DE PRÉTRAITEMENT D'URINE ET PROCÉDÉ DE DÉTERMINATION DE PROTÉINE URINAIRE
(JA) 尿中タンパク質定量用の尿前処理剤、尿前処理方法、及び尿中タンパク質定量方法。
Abstract
(EN)
It is intended to develop a urine pretreatment agent which is capable of reducing or eliminating an effect of pH variation in urine, eliminating an effect of a deposit resulting from deposition of an inorganic salt in the urine, and solubilizing a membrane protein; a urine pretreatment method; and a urinary protein determination method. A urine pretreatment agent for urinary protein determination characterized by containing a buffer, a chelator, and a surfactant; a urine pretreatment method characterized by mixing the urine pretreatment agent in an amount of 10 to 1000 parts by mass based on 100 parts by mass of urine; and a urinary proteindetermination method characterized by mixing the urine pretreatment agent in an amount of 10 to 1000 parts by mass based on 100 parts by mass of urine and thereafter measuring a protein concentration are provided.
(FR)
L'invention vise à développer un agent de prétraitement d'urine qui est capable de réduire ou d'éliminer un effet d'une variation de pH dans l'urine, éliminant un effet d'un dépôt résultant d'une déposition d'un sel inorganique dans l'urine, et de rendre soluble une protéine membranaire ; un procédé de prétraitement d'urine et un procédé de détermination de protéine urinaire. L'invention porte sur un agent de prétraitement urinaire pour détermination de protéine urinaire, qui est caractérisé par le fait qu'il contient un tampon, un agent chélatant et un agent tensio-actif ; sur un procédé de prétraitement d'urine qui est caractérisé par le mélange de l'agent de prétraitement d'urine dans une quantité de 10 à 1 000 parties en masse sur la base de 100 parties en masse d'urine ; et sur un procédé de détermination de protéine urinaire caractérisé par le mélange de l'agent de prétraitement d'urine dans une quantité de 10 à 1 000 parties en masse sur la base de 100 parties en masse d'urine et, par la suite, la mesure d'une concentration de protéine.
(JA)
尿のpHの変動の影響を低減又は解消し、尿中の無機塩が析出した沈降物の影響を解消し、膜タンパク質の可溶化ができる尿前処理剤、尿前処理方法、及び尿中タンパク質定量方法を開発すること。 緩衝剤、キレート剤及び界面活性剤を含むことを特徴とする尿中タンパク質定量用の尿前処理剤と、尿100質量部に対して前記尿前処理剤10~1000質量部を混合することを特徴とする尿前処理方法と、尿100質量部に対して前記尿前処理剤10~1000質量部を混合した後、タンパク質濃度を測定することを特徴とする尿中タンパク質定量方法とを提供する。
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