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1. WO2009007993 - A PROCESS FOR PURIFICATION OF IMMUNOGLOBULINS USING A PSEUDOBIOAFFINITY ADSORBENT

Publication Number WO/2009/007993
Publication Date 15.01.2009
International Application No. PCT/IN2008/000254
International Filing Date 23.04.2008
Chapter 2 Demand Filed 23.03.2009
IPC
C07K 16/06 2006.01
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
16Immunoglobulins, e.g. monoclonal or polyclonal antibodies
06from serum
CPC
C07K 16/065
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
16Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
06from serum
065Purification, fragmentation
Y10T 428/249991
YSECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
10TECHNICAL SUBJECTS COVERED BY FORMER USPC
TTECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
428Stock material or miscellaneous articles
249921Web or sheet containing structurally defined element or component
249953Composite having voids in a component [e.g., porous, cellular, etc.]
249987With nonvoid component of specified composition
249991Synthetic resin or natural rubbers
Y10T 428/249992
YSECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
10TECHNICAL SUBJECTS COVERED BY FORMER USPC
TTECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
428Stock material or miscellaneous articles
249921Web or sheet containing structurally defined element or component
249953Composite having voids in a component [e.g., porous, cellular, etc.]
249987With nonvoid component of specified composition
249991Synthetic resin or natural rubbers
249992Linear or thermoplastic
Applicants
  • LALI, Arvind Mallinath [IN]/[IN]
Inventors
  • LALI, Arvind Mallinath
  • NAIK, Amith, Dattatray
  • RAINA, Monika
  • KALE, Sandeep, Bhaskar
Agents
  • LALI, Arvind, Mallinath
Priority Data
635/mum/200623.04.2007IN
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) A PROCESS FOR PURIFICATION OF IMMUNOGLOBULINS USING A PSEUDOBIOAFFINITY ADSORBENT
(FR) PROCÉDÉ DE PURIFICATION D'IMMUNOGLOBULINES AU MOYEN D'UN ADSORBANT FONCTIONNANT PAR PSEUDOBIOAFFINITÉ
Abstract
(EN)
The present invention relates to the development of pseudobioaffinity adsorbent and process for purification of immunoglobulin G from immunoglobulin containing solutions such as but not limited to plasma, serum, cell culture supernatant, ascites fluids. The adsorbent consists of a solid support and ligand. The ligand may be attached to the matrix or be part of matrix. The ligand is selected from a group of hydrophobic amino acids such as alanine, valine, leucine, isoleucine, proline, phenylalanine, tryptophan and tyrosine and the support material being preferably a synthetic hydrophilic polymer of methacrylate or acrylate species, or any of its derivatives. The adsorbent is cheap and stable to harsh conditions such as 1.0 M NaOH used during regeneration of adsorbents. Moreover there is no problem of toxic leachables typically associated with biological ligands. The nature of the adsorbent also allows high flow rate operations at relatively low pressures. The process includes adjusting the pH and conductivity of the feed solution and the use of ionic salts and additives such as polyols or alcohols for eluting the bound IgG.
(FR)
La présente invention concerne la mise au point d'un adsorbant fonctionnant par pseudobioaffinité et d'un procédé de purification d'immunoglobulines G à partir de solutions contenant des immunoglobulines, par exemple, mais la liste n'est pas limitative, de plasma, de sérum, de surnageant de cultures cellulaires, de liquides d'ascites. L'adsorbant est constitué d'un support solide et d'un ligand. Le ligand peut être fixé à la matrice ou faire partie de la matrice. Le ligand est choisi dans un groupe constitué d'acides aminés hydrophobes comme l'alanine, la valine, la leucine, l'isoleucine, la proline, la phénylalanine, le tryptophane et la tyrosine et le matériau servant de support est, de préférence, un polymère hydrophile de synthèse à base de méthacrylate ou d'acrylate, ou l'un quelconque de ses dérivés. L'adsorbant est bon marché et stable même dans les conditions les plus rigoureuses, par exemple en présence de NaOH 1,0 M utilisé lors de la régénération des adsorbants. En outre, il n'existe pas de problèmes de toxicité des produits de lixiviation typiquement associés aux ligands biologiques. La nature de l'adsorbant permet également un fonctionnement caractérisé par une vitesse d'écoulement élevée à des pressions relativement basses. Le procédé comprend l'ajustement du pH et de la conductivité de la solution servant à l'alimentation du processus et l'utilisation de sels ioniques et d'additifs, tels que les polyols ou les alcools, en vue de l'élution des IgG liées.
Also published as
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