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1. WO2009003645 - SYSTEMS AND METHODS FOR DETERMINING CROSS-TALK COEFFICIENTS IN PCR AND OTHER DATA SETS

Publication Number WO/2009/003645
Publication Date 08.01.2009
International Application No. PCT/EP2008/005255
International Filing Date 27.06.2008
Chapter 2 Demand Filed 09.02.2009
IPC
G01N 21/64 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible or ultra-violet light
62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
63optically excited
64Fluorescence; Phosphorescence
G01N 21/27 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible or ultra-violet light
17Systems in which incident light is modified in accordance with the properties of the material investigated
25Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
27using photo-electric detection
G06K 9/00 2006.01
GPHYSICS
06COMPUTING; CALCULATING OR COUNTING
KRECOGNITION OF DATA; PRESENTATION OF DATA; RECORD CARRIERS; HANDLING RECORD CARRIERS
9Methods or arrangements for reading or recognising printed or written characters or for recognising patterns, e.g. fingerprints
CPC
C12Q 1/6851
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6844Nucleic acid amplification reactions
6851Quantitative amplification
Applicants
  • ROCHE DIAGNOSTICS GMBH [DE]/[DE] (DE)
  • F.HOFFMANN-LA ROCHE AG [CH]/[CH] (AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BE, BF, BG, BH, BJ, BR, BW, BY, BZ, CA, CF, CG, CH, CI, CM, CN, CO, CR, CU, CY, CZ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, FR, GA, GB, GD, GE, GH, GM, GN, GQ, GR, GT, GW, HN, HR, HU, ID, IE, IL, IN, IS, IT, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LV, LY, MA, MC, MD, ME, MG, MK, ML, MN, MR, MT, MW, MX, MY, MZ, NA, NE, NG, NI, NL, NO, NZ, OM, PG, PH, PL, PT, RO, RS, RU, SC, SD, SE, SG, SI, SK, SL, SM, SN, SV, SY, SZ, TD, TG, TJ, TM, TN, TR, TT, TZ, UA, UG, UZ, VC, VN, ZA, ZM, ZW)
  • KURNIK, Ronald, T. [US]/[US] (UsOnly)
  • SANE, Aditya [IN]/[US] (UsOnly)
  • ELKIN, Chris [US]/[US] (UsOnly)
Inventors
  • KURNIK, Ronald, T.
  • SANE, Aditya
  • ELKIN, Chris
Common Representative
  • ROCHE DIAGNOSTICS GMBH
Priority Data
60/947,06529.06.2007US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) SYSTEMS AND METHODS FOR DETERMINING CROSS-TALK COEFFICIENTS IN PCR AND OTHER DATA SETS
(FR) SYSTÈMES ET PROCÉDÉS POUR DÉTERMINER DES COEFFICIENTS DE CROSS-TALK (CHEVAUCHEMENT) DANS DES JEUX DE DONNÉES PCR ET AUTRES
Abstract
(EN)
Systems and methods for determining cross-talk coefficients in curves, such as sigmoid-type or growth curves, and PCR curves and nucleic acid melting curves in particular, as well as for applying the cross-talk coefficients to produce cross-talk corrected data sets using a linear subtractive model. Cross-talk signal coefficients are determined using cross-talk data acquired across the entire signal acquisition range. Analyzing across all of the signal curve data provides for a more robust cross-talk correction across the entire data acquisition range. A linear subtractive model is used to correct data sets having cross-talk components.
(FR)
La présente invention concerne des systèmes et des procédés pour déterminer des coefficients de cross-talk (chevauchement) dans des courbes, telles que des courbes de type sigmoïde ou de croissance, et en particulier des courbes PCR et des courbes de fusion d'acides nucléiques, ainsi que pour appliquer les coefficients de cross-talk (chevauchement) afin de produire des jeux de données de cross-talk (chevauchement) corrigées au moyen d'un modèle linéaire soustractif. Les coefficients de signaux de cross-talk (chevauchement) sont déterminés au moyen de données de cross-talk (chevauchement) acquises sur la totalité de la plage d'acquisition de signaux. L'analyse de la totalité des données de la courbe de signaux fournit une correction de cross-talk (chevauchement) plus robuste sur la totalité de la plage d'acquisition de données. Un modèle linéaire soustractif est utilisé pour corriger les jeux de données ayant des composantes de cross-talk (chevauchement).
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