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Machine translation
1. (WO2008129554) STEM CELL-DERIVED RETINAL PIGMENT EPITHELIAL CELLS
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/2008/129554    International Application No.:    PCT/IL2008/000556
Publication Date: 30.10.2008 International Filing Date: 27.04.2008
IPC:
A61K 35/44 (2006.01), C12N 5/071 (2010.01)
Applicants: HADASIT MEDICAL RESEARCH SERVICES & DEVELOPMENT LIMITED [IL/IL]; P.O.Box 12000 Kiryat Hadassah 91120 Jerusalem (IL) (For All Designated States Except US).
IDELSON, Masha [IL/IL]; (IL) (For US Only).
ALPER-PINUS, Ruslana [IL/IL]; (IL) (For US Only).
OBOLENSKY, Alex [IL/IL]; (IL) (For US Only).
BANIN, Eyal [IL/IL]; (IL) (For US Only).
REUBINOFF, Benjamin [IL/IL]; (IL) (For US Only)
Inventors: IDELSON, Masha; (IL).
ALPER-PINUS, Ruslana; (IL).
OBOLENSKY, Alex; (IL).
BANIN, Eyal; (IL).
REUBINOFF, Benjamin; (IL)
Agent: REINHOLD COHN AND PARTNERS; P.O.Box 4060 61040 Tel Aviv (IL)
Priority Data:
60/907,818 18.04.2007 US
Title (EN) STEM CELL-DERIVED RETINAL PIGMENT EPITHELIAL CELLS
(FR) CELLULES DE L'ÉPITHÉLIUM PIGMENTAIRE DE LA RÉTINE DÉRIVÉES DE CELLULES SOUCHES
Abstract: front page image
(EN)The present invention concerns RPE cells obtainable by directed differentiation from stem cell, particularly, human stem cells. It has been specifically found that culturing stem cells in the presence of one or more member of the TGF superfamily, such as Activin A) induced directed differentiation into mature and functional RPE cells. This was evidenced by the expression of markers specific to mature RPE cells, including MiTF-A, RPE65 or Bestrophin). In accordance with one particular embodiment, the cells are a priori cultured with nicotinamide (NA) which was found to augment the cells' response to the inductive effect of the one or more member of the TGF superfamily. The invention also provides methods of performing the directed differentiation, as well as methods for use of the resulting RPE cells.
(FR)Cette invention a trait à des cellules EPR qui peuvent être obtenues par différentiation orientée à partir de cellules souches, en particulier de cellules souches humaines. Des données ont montré spécifiquement que la mise en culture de cellules souches, en présence d'un ou de plusieurs membres de la superfamille des TGF, comme l'activine A) induit une différentiation orientée en cellules EPR matures et fonctionnelles. Ces données ont été mises en évidence par l'expression de marqueurs spécifiques des cellules EPR matures, y compris le MiTF-A, le RPE65 ou la bestrophine. Conformément à un mode de réalisation particulier, les cellules sont d'abord cultivées avec du nicotinamide (NA) qui s'avère augmenter la réponse cellulaire à l'effet inducteur d'un ou de plusieurs membres de la superfamille des TGF. L'invention concerne également des procédés de réalisation de la différentiation orientée, ainsi que des procédés utilisant les cellules EPR résultantes.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BR, BW, BY, BZ, CA, CH, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PG, PH, PL, PT, RO, RS, RU, SC, SD, SE, SG, SK, SL, SM, SV, SY, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW.
African Regional Intellectual Property Organization (BW, GH, GM, KE, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MT, NL, NO, PL, PT, RO, SE, SI, SK, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG).
Publication Language: English (EN)
Filing Language: English (EN)