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1. (WO2008120218) UNDIFFERENTIATED STEM CELL CULTURE SYSTEMS
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/2008/120218    International Application No.:    PCT/IL2008/000460
Publication Date: 09.10.2008 International Filing Date: 02.04.2008
IPC:
C12N 5/0735 (2010.01)
Applicants: HADASIT MEDICAL RESEARCH SERVICES & DEVELOPMENT LIMITED [IL/IL]; P.O.Box 12000 Kiryat Hadassah 91120 Jerusalem (IL) (For All Designated States Except US).
REUBINOFF, Benjamin [IL/IL]; (IL) (For US Only).
STEINER, Debora [IL/IL]; (IL) (For US Only)
Inventors: REUBINOFF, Benjamin; (IL).
STEINER, Debora; (IL)
Agent: REINHOLD COHN AND PARTNERS; P.O.Box 4060, 61040 Tel Aviv (IL)
Priority Data:
11/730,560 02.04.2007 US
Title (EN) UNDIFFERENTIATED STEM CELL CULTURE SYSTEMS
(FR) SYSTÈMES DE CULTURE DE CELLULES SOUCHES NON DIFFÉRENCIÉES
Abstract: front page image
(EN)The present disclosure provides methods for maintaining and propagating undifferentiated pluripotent stem cells (SC) in suspension. The methods comprise culturing such SC in a non-adherent culture dish under conditions comprising a basic serum free medium and one or more of a basic medium, a serum replacement, an extra cellular matrix component and a factor supporting expansion of said SC. A specific and preferred culture condition comprise supplementing Neurobasal™ medium with KO serum replacement (KOSR). These conditions allowed for large scale and long term propagation of undifferentiated pluripotent SC. The culture system comprising suspended undifferentiated pluripotent SC were found to have many applications including in methods for directed as well as spontaneous differentiation of the SC into somatic cells. Also disclosed herein is a method of deriving SC, preferably human embryonic SC from human embryos via the formation of cell clusters.
(FR)La présente invention concerne des procédés d'entretien et de propagation de cellules souches (SC) pluripotentes non différenciées en suspension. Les procédés comprennent la culture de telles cellules souches dans un bac de culture non adhérent dans des conditions qui comprennent un milieu basique sans sérum et un milieu basique et/ou un substitut de sérum et/ou un composant de matrice extra cellulaire et/ou un facteur favorisant l'expansion desdites cellules souches. Une condition de culture spécifique et préférée comprend un milieu d'enrichissement Neurobasal™ avec substitut de sérum KO (KOSR). Ces conditions permettent la propagation à long terme et à grande échelle de cellules souches pluripotentes non différenciées. Les systèmes de culture comprenant des cellules souches pluripotentes non différenciées en suspension se sont révélés comme possédant de nombreuses applications, notamment dans des procédés de différenciation aussi bien dirigée que spontanée de cellules souches en cellules somatiques. L'invention concerne également un procédé destiné à dériver des cellules souches, de préférence des cellules souches embryonnaires humaines, à partir d'embryons humains par formation d'agrégats cellulaires.
Designated States: AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BR, BW, BY, BZ, CA, CH, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PG, PH, PL, PT, RO, RS, RU, SC, SD, SE, SG, SK, SL, SM, SV, SY, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW.
African Regional Intellectual Property Organization (BW, GH, GM, KE, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MT, NL, NO, PL, PT, RO, SE, SI, SK, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG).
Publication Language: English (EN)
Filing Language: English (EN)