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1. WO2008080124 - BUTANOL PRODUCTION BY METABOLICALLY ENGINEERED YEAST

Publication Number WO/2008/080124
Publication Date 03.07.2008
International Application No. PCT/US2007/088705
International Filing Date 21.12.2007
IPC
C12P 7/16 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
7Preparation of oxygen-containing organic compounds
02containing a hydroxy group
04acyclic
16Butanols
CPC
C12N 9/0008
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes; Proenzymes; Compositions thereof
0004Oxidoreductases (1.)
0008acting on the aldehyde or oxo group of donors (1.2)
C12N 9/88
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes; Proenzymes; Compositions thereof
88Lyases (4.)
C12N 9/93
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes; Proenzymes; Compositions thereof
93Ligases (6)
C12P 7/16
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
7Preparation of oxygen-containing organic compounds
02containing a hydroxy group
04acyclic
16Butanols
Y02E 50/10
YSECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
EREDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
50Technologies for the production of fuel of non-fossil origin
10Biofuels, e.g. bio-diesel
Applicants
  • GEVO, INC. [US]/[US] (AllExceptUS)
  • GUNAWARDENA, Uvini [LK]/[US] (UsOnly)
  • MEINHOLD, Peter [DE]/[US] (UsOnly)
  • PETERS, Matthew W. [US]/[US] (UsOnly)
  • URANO, Jun [US]/[US] (UsOnly)
  • FELDMAN, Reid M. Renny [US]/[US] (UsOnly)
Inventors
  • GUNAWARDENA, Uvini
  • MEINHOLD, Peter
  • PETERS, Matthew W.
  • URANO, Jun
  • FELDMAN, Reid M. Renny
Agents
  • VEITENHEIMER, Erich
Priority Data
60/871,42721.12.2006US
60/888,01602.02.2007US
60/928,28308.05.2007US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) BUTANOL PRODUCTION BY METABOLICALLY ENGINEERED YEAST
(FR) PRODUCTION DE BUTANOL PAR UNE LEVURE MÉTABOLIQUEMENT MODIFIÉE
Abstract
(EN)
There are disclosed metabolically-engineered yeast and methods of producing n-butanol. In an embodiment, metabolically-engineered yeast is capable of metabolizing a carbon source to produce n-butanol, at least one pathway produces increased cytosolic acetyl-CoA relative to cytosolic acetyl-CoA produced by a wild-type yeast, and at least one heterologous gene encodes and expresses at least one enzyme for a metabolic pathway capable of utilizing NADH to convert acetyl-CoA to n-butanol. In another embodiment, a method of producing n-butanol includes (a) providing metabolically-engineered yeast capable of metabolizing a carbon source to produce n-butanol, at least one pathway produces increased cytosolic acetyl-CoA relative to cytosolic acetyl-CoA produced by a wild-type yeast, and at least one heterologous gene encodes and expresses at least one enzyme for a metabolic pathway utilizing NADH to convert acetyl-CoA to n-butanol; and (b) culturing the yeast to produce n-butanol. Other embodiments are also disclosed.
(FR)
L'invention concerne une levure métaboliquement modifiée et des procédés de production de n-butanol. Dans un mode de réalisation, une levure métaboliquement modifiée est capable de métaboliser une source de carbone pour produire du n-butanol, au moins une voie produisant de l'acétyl-CoA cytosolique amélioré par rapport à l'acétyl-CoA cytosolique produit par une levure sauvage et au moins un gène xénogénique codant et exprimant au moins une enzyme pour une voie métabolique capable d'utiliser NADH pour convertir l'acétyl-CoA en n-butanol. Dans un autre mode de réalisation, un procédé de production de n-butanol comprend (a) la formation d'une levure métaboliquement modifiée capable de métaboliser une source de carbone pour produire du n-butanol, au moins une voie produisant de l'acétyl-CoA cytosolique amélioré par rapport à l'acétyl-CoA cytosolique produit par une levure sauvage et au moins un gène xénogénique codant et exprimant au moins une enzyme pour une voie métabolique utilisant NADH pour convertir l'acétyl-CoA en n-butanol ; et (b) la mise en culture de la levure pour produire du n-butanol. D'autres modes de réalisation sont également décrits.
Also published as
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