WO2008040599 PROCESS FOR THE PRODUCTION OF RECOMBINANT PROTEINS USING CARNIVOROUS PLANTS.

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Publication Number WO/2008/040599

Publication Date 10.04.2008

International Application No. PCT/EP2007/058950

International Filing Date 29.08.2007

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Applicants

PLANT ADVANCED TECHNOLOGIES PAT SAS [FR]/[FR] (AllExceptUS)
BITEAU, Flore [FR]/[FR] (UsOnly)
BOURGAUD, Frédéric [FR]/[FR] (UsOnly)
GONTIER, Eric [FR]/[FR] (UsOnly)
FEVRE, Jean-Paul [FR]/[FR] (UsOnly)

Inventors

BITEAU, Flore
BOURGAUD, Frédéric
GONTIER, Eric
FEVRE, Jean-Paul

Agents

WARCOIN, AHNER, TEXIER, LE FORESTIER, CALLON DE LAMARCK, COLLIN, TETAZ-Cabinet Regimbeau

Priority Data

06/08693		04.10.2006		FR
60/856,784		06.11.2006		US

Publication Language English (en)

Filing Language English (EN)

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Title

(EN) PROCESS FOR THE PRODUCTION OF RECOMBINANT PROTEINS USING CARNIVOROUS PLANTS.

(FR) PROCÉDÉ DE PRODUCTION DE PROTÉINES RECOMBINANTES AU MOYEN DE PLANTES CARNIVORES

Abstract

(EN) The present application relates to the provision of process for producing at least one protein, comprising the cultivation of a carnivorous plant, characterized in that said plant has been genetically modified to express said protein or proteins, and said protein or proteins are collected from the digestive secretions of said carnivorous plant traps, in particular glue, pitcher, trumpet or bladder traps. The proteins of interest are functional, despite the existence of digestive enzymes.

(FR) La présente invention concerne un procédé de production d'au moins une protéine. Le procédé consiste à cultiver une plante carnivore et il est caractérisé en ce que ladite plante a été génétiquement modifiée pour exprimer ladite protéine ou lesdites protéines. Ladite protéine ou lesdites protéines sont recueillies dans les sécrétions digestives des pièges de ladite plante carnivore, en particulier des pièges à colle, à urne, en trompette et en forme de vessie. Les protéines d'intérêt sont fonctionnelles, en dépit de l'existence d'enzymes digestives.

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N	MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15	Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09	Recombinant DNA-technology
63	Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79	Vectors or expression systems specially adapted for eukaryotic hosts
82	for plant cells

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N	MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15	Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09	Recombinant DNA-technology
63	Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79	Vectors or expression systems specially adapted for eukaryotic hosts
82	for plant cells

A	HUMAN NECESSITIES
61	MEDICAL OR VETERINARY SCIENCE; HYGIENE
P	SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
43	Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N	MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15	Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09	Recombinant DNA-technology
63	Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79	Vectors or expression systems specially adapted for eukaryotic hosts
82	for plant cells ; , e.g. plant artificial chromosomes (PACs)
8201	Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
8202	by biological means, e.g. cell mediated or natural vector
8205	Agrobacterium mediated transformation

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N	MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15	Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09	Recombinant DNA-technology
63	Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79	Vectors or expression systems specially adapted for eukaryotic hosts
82	for plant cells ; , e.g. plant artificial chromosomes (PACs)
8241	Phenotypically and genetically modified plants via recombinant DNA technology
8242	with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits

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1. WO2008040599 - PROCESS FOR THE PRODUCTION OF RECOMBINANT PROTEINS USING CARNIVOROUS PLANTS.