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Machine translation
1. (WO2008039998) METHODS FOR SEQUENCING DNA
Latest bibliographic data on file with the International Bureau   

Pub. No.:    WO/2008/039998    International Application No.:    PCT/US2007/079936
Publication Date: 03.04.2008 International Filing Date: 28.09.2007
IPC:
C12Q 1/68 (2006.01), C12P 19/34 (2006.01), C12M 1/34 (2006.01), C07H 21/02 (2006.01)
Applicants: PRESIDENT AND FELLOWS OF HARVARD COLLEGE [US/US]; 17 Quincy Street, Cambridge, MA 02138 (US) (For All Designated States Except US).
THE BRIGHAM AND WOMEN'S HOSPITAL, INC. [US/US]; 75 Francis Street, Boston, MA 02115 (US) (For All Designated States Except US).
KIM, Jae, Bum [US/US]; (US) (For US Only).
PORRECA, Gregory, J. [US/US]; (US) (For US Only).
CHURCH, George, M. [US/US]; (US) (For US Only).
SEIDMAN, Jonathan, G. [US/US]; (US) (For US Only)
Inventors: KIM, Jae, Bum; (US).
PORRECA, Gregory, J.; (US).
CHURCH, George, M.; (US).
SEIDMAN, Jonathan, G.; (US)
Agent: IWANICKI, John, P.; Banner & Witcoff, Ltd., 28 State Street, 28th Floor, Boston, MA 02109 (US)
Priority Data:
60/847,752 28.09.2006 US
Title (EN) METHODS FOR SEQUENCING DNA
(FR) PROCÉDÉS DE SÉQUENÇAGE D'ADN
Abstract: front page image
(EN)The invention is directed to methods for using sequence by ligation to sequence DNA immobilized on miniaturized, high density bead-based arrays. Methods are provided to fabricate an array of beads where the beads are coupled directly to a solid support. Methods are also provided to improve signal and reduce background in ligation-mediated DNA sequencing. In addition, methods are provided to improve the accuracy of reported tag counts when performing DNA sequencing by fluorescent nonamer ligation.
(FR)L'invention a pour objet des procédés permettant d'utiliser une séquence par ligature à une séquence d'ADN immobilisée sur des réseaux de billes miniaturisés haute densité. Des procédés sont fournis pour fabriquer un réseau de billes dans lequel les billes sont couplées directement à un support solide. Des procédés sont aussi fournis pour améliorer un signal et réduire l'arrière-plan dans un séquençage d'ADN régulé par ligature. De plus, des procédés sont fournis pour améliorer la précision des décomptes d'étiquette reportés lors de la réalisation d'un séquençage d'ADN par une ligature nonamère fluorescente.
Designated States: AE, AG, AL, AM, AT, AU, AZ, BA, BB, BG, BH, BR, BW, BY, BZ, CA, CH, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IS, JP, KE, KG, KM, KN, KP, KR, KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PG, PH, PL, PT, RO, RS, RU, SC, SD, SE, SG, SK, SL, SM, SV, SY, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW.
African Regional Intellectual Property Organization (BW, GH, GM, KE, LS, MW, MZ, NA, SD, SL, SZ, TZ, UG, ZM, ZW)
Eurasian Patent Organization (AM, AZ, BY, KG, KZ, MD, RU, TJ, TM)
European Patent Office (AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HU, IE, IS, IT, LT, LU, LV, MC, MT, NL, PL, PT, RO, SE, SI, SK, TR)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, ML, MR, NE, SN, TD, TG).
Publication Language: English (EN)
Filing Language: English (EN)