Processing

Please wait...

Settings

Settings

Goto Application

1. WO2008024447 - DIFFERENTIATION OF PROGENITOR CELLS INTO FIBROBLASTS

Publication Number WO/2008/024447
Publication Date 28.02.2008
International Application No. PCT/US2007/018670
International Filing Date 22.08.2007
IPC
C12N 5/00 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
C12N 5/02 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
02Propagation of single cells or cells in suspension; Maintenance thereof; Culture media therefor
CPC
C12N 2501/10
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
2501Active agents used in cell culture processes, e.g. differentation
10Growth factors
C12N 5/0656
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor;
06Animal cells or tissues; Human cells or tissues
0602Vertebrate cells
0652Cells of skeletal and connective tissues; Mesenchyme
0656Adult fibroblasts
Applicants
  • THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OFNEW YORK [US]/[US] (AllExceptUS)
  • MAO, Jeremy [CA]/[US] (UsOnly)
Inventors
  • MAO, Jeremy
Agents
  • BOCK, Joel, N.
Priority Data
60/823,25022.08.2006US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) DIFFERENTIATION OF PROGENITOR CELLS INTO FIBROBLASTS
(FR) DIFFÉRENCIATION DE CELLULES PROGÉNITRICES DANS DES FIBROBLASTES
Abstract
(EN)
Disclosed herein is a new approach towards differentiation of progenitor cells, for example human mesenchymal cells (hMSCs), into fibroblasts using chemical factors, such as by the treatment of recombinant human connective tissue growth factor (CTGF) and ascorbic acid. One aspect of the invention provides an ex vivo culturing protocol for fibroblastic differentiation of tissue progenitor cells (e.g., human mesenchymal stem cells (hMSCs)) using bioactive factors such as connective tissue growth factor (CTGF). Approaches described herein can provide for significant increases in collagen production from cultured fibroblast progenitor cells.
(FR)
L'invention concerne une nouvelle approche vers la différenciation de cellules progénitrices, par exemple de cellules mésenchymateuses humaines (hMSC), dans des fibroblastes à l'aide de facteurs chimiques, par exemple par le traitement du facteur de croissance du tissu conjonctif humain recombinant (CTGF) et l'acide ascorbique. Un aspect de l'invention concerne un protocole de mise en culture ex vivo pour la différenciation fibroblastique de cellules progénitrices de tissu (par exemple, de cellules souches mésenchymateuses humaines (hMSC)) à l'aide de facteurs bioactifs tels que le facteur de croissance du tissu conjonctif (CTGF). Les approches décrites présentement peuvent conduire à des augmentations significatives de la production de collagène à partir de cellules progénitrices de fibroblastes mises en culture.
Latest bibliographic data on file with the International Bureau