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1. WO2008019448 - TIME GATED FLUORESCENT FLOW CYTOMETER

Publication Number WO/2008/019448
Publication Date 21.02.2008
International Application No. PCT/AU2007/001168
International Filing Date 17.08.2007
IPC
G01N 21/64 2006.1
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible or ultra-violet light
62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
63optically excited
64Fluorescence; Phosphorescence
G01N 15/14 2006.1
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
15Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
10Investigating individual particles
14Electro-optical investigation
CPC
G01N 15/14
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
15Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
10Investigating individual particles
14Electro-optical investigation, e.g. flow cytometers
G01N 15/1434
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
15Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
10Investigating individual particles
14Electro-optical investigation, e.g. flow cytometers
1434using an analyser being characterised by its optical arrangement
G01N 15/1436
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
15Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
10Investigating individual particles
14Electro-optical investigation, e.g. flow cytometers
1434using an analyser being characterised by its optical arrangement
1436the optical arrangement forming an integrated apparatus with the sample container, e.g. a flow cell
Applicants
  • MACQUARIE UNIVERSITY [AU]/[AU] (AllExceptUS)
  • DAYONG, Jin [AU]/[AU] (UsOnly)
  • PIPER, Jim [AU]/[AU] (UsOnly)
  • CONNALLY, Russell [AU]/[AU] (UsOnly)
Inventors
  • DAYONG, Jin
  • PIPER, Jim
  • CONNALLY, Russell
Agents
  • BLAKE DAWSON WALDRON PATENT SERVICES
Priority Data
200690452518.08.2006AU
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) TIME GATED FLUORESCENT FLOW CYTOMETER
(FR) CYTOMÈTRE EN FLUX FLUORESCENT À DÉCLENCHEMENT PÉRIODIQUE
Abstract
(EN)
An apparatus (10) for detecting a particle (12) labelled with a fluorescent marker is disclosed. The apparatus (10) has a flow cell (16) being adapted to contain a fluid (14) in which the particle (12) is suspended. A light source (28) is operatively coupled to the flow cell (16) and arranged for emitting a stimulating light (28) which is effective in optically exciting the fluorescent marker (12) for emitting a fluorescent light (30). The apparatus (10) also includes a spatial filter (50) across an optical path between the particle (12) and a time gated detector (32) operatively coupled to the flow cell (16) for detecting the fluorescent light (30).
(FR)
La présente invention concerne un appareil (10) destiné à détecter une particule (12) marquée avec un marqueur fluorescent. L'appareil (10) comprend une cellule de flux (16) conçue pour contenir un fluide (14) dans lequel la particule (12) se trouve en suspension. Une source lumineuse (28) est couplée fonctionnellement à la cellule de flux (16) et conçue pour émettre une lumière stimulante (28) qui est efficace en termes d'excitation optique du marqueur fluorescent (12) pour qu'il émette une lumière fluorescente (30). L'appareil (10) comprend également un filtre spatial (50) sur un trajet optique entre la particule (12) et un détecteur à déclenchement périodique (32) couplé fonctionnellement à la cellule de flux (16) de manière à détecter la lumière fluorescente (30).
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