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1. WO2007149946 - COMPOSITION AND METHODS FOR EXPRESSING REPORTER MOLECULES IN MAMMALIAN CELLS

Publication Number WO/2007/149946
Publication Date 27.12.2007
International Application No. PCT/US2007/071704
International Filing Date 20.06.2007
IPC
C12N 15/33 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
11DNA or RNA fragments; Modified forms thereof
31Genes encoding microbial proteins, e.g. enterotoxins
33Genes encoding viral proteins
CPC
C12N 15/86
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
85for animal cells
86Viral vectors
C12N 2710/14143
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
2710dsDNA Viruses
00011dsDNA Viruses
14011Baculoviridae
14111Nucleopolyhedrovirus, e.g. autographa californica nucleopolyhedrovirus
14141Use of virus, viral particle or viral elements as a vector
14143viral genome or elements thereof as genetic vector
C12N 2830/85
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
2830Vector systems having a special element relevant for transcription
80from vertebrates
85mammalian
Applicants
  • INVITROGEN CORPORATION [US]/[US] (AllExceptUS)
  • HANSON, George [US]/[US] (UsOnly)
  • THOMPSON, David [US]/[US] (UsOnly)
Inventors
  • HANSON, George
  • THOMPSON, David
Agents
  • FOILES, Peter
Priority Data
60/805,29720.06.2006US
60/889,91314.02.2007US
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) COMPOSITION AND METHODS FOR EXPRESSING REPORTER MOLECULES IN MAMMALIAN CELLS
(FR) COMPOSITIONS ET PROCÉDÉS POUR EXPRIMER DES MOLÉCULES RAPPORTEURS DANS DES CELLULES DE MAMMIFÈRES
Abstract
(EN)
Disclosed herein is a novel system and methods for expressing exogenous genes, such as genes encoding fluorescent proteins, in mammalian cells. In one embodiment of this system and methods, a gene essential for viral infectivity or replication in cell culture is deleted or inactivated in the genome of a non-mammaian DNA virus. The exogenous gene operably linked to a mammalian promoter is then inserted into the non-replicative non-mammalian DNA virus. The non-replicative virus is propagated in a host cell that expresses in trans the deleted or inactivated gene or a functional homolog.
(FR)
L'invention concerne un nouveau système et des procédés pour exprimer des gènes exogènes, tels que des gènes codant pour des protéines fluorescentes, dans des cellules de mammifères. Dans un mode de réalisation de ce système et de ces procédés, un gène essentiel pour l'infectivité virale ou la réplication en culture cellulaire est délété ou inactivé dans le génome d'un virus d'ADN non mammifère. Le gène exogène lié de façon fonctionnelle à un promoteur de mammifère est ensuite introduit dans le virus d'ADN non mammifère non réplicatif. Le virus non réplicatif est propagé dans une cellule hôte qui exprime en trans le gène délété ou inactivé ou un homologue fonctionnel.
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