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1. WO2007149343 - PROTEASES FOR TREATMENT OF VENOMOUS BITES

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[ EN ]

CLAIMS
What is claimed is:
1. A preparation for treating venom toxicity comprising a protease polypeptide in a stable, injectable formulation, wherein said protease is one that cleaves a protein toxin of the venom.
2. The preparation of claim 1 wherein the protease is a mast cell protease.
3. The preparation of claim 1 or 2 wherein the protease is a human protease.
4. The preparation of claiml wherein the protease is selected from the group consisting of chymotrypsin, papain and carboxypeptidase B.
5. The preparation of claim 1 wherein the protease is selected from the group consisting of carboxypeptidase A, tryptase and chymase.
6. The preparation of claim 1 wherein the protease comprises a carboxypeptidase.
7. The preparation of claim 6 wherein the carboxypeptidase is human carboxypeptidase A.

8. The preparation of claim 1 wherein the carboxypeptidase has at least 80% sequence identity to the amino acid sequence of human carboxypeptidase A.
9. The preparation of claim 1 or 2 wherein the protease is in the form of a proenzyme.

10. The preparation of claim 1 wherein the protease is in a stable liquid preparation.
11. The preparation of claim 1 wherein the protease is a lyophilized powder, and further containing sterile diluent for reconstituting the powder.
12. The preparation of claim 10 or 11 further comprised in a kit comprising a syringe for injecting the protease.
13. The preparation of claim 12 wherein at least one protease is selected from the group consisting of chymotrypsin, papain, carboxypeptidase B, carboxypeptidase A, tryptase and chymase.
14. The preparation of claim 1 comprising at least two different proteases.
15. The preparation of claim 1, 2, or 3 where the protease is selected to cleave at least one of sarafotoxin 6b, Atractaspis engaddensis venom, Agkistrodon contortrix contortrix venom Crotalus atrox venom, dendrotoxin, and Loxosceles recluse venom.

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16. A method for treating a venomous bite comprising injecting the bite victim with material having protease activity against a venom protein.
17. The method of claim 16 wherein the material having protease activity is a mast cell protease
18. The method of claim 16 wherein the protease is selected from the group consisting of carboxypeptidase A, chymotrypsin, carboxypeptidase B, tryptase and chymase.
19. The method of claim 16 wherein the material has carboxypetidase activity against the venom.
20. The method of claim 16 where the venom protein is one selected from Table 1 and Table 2.
21. The method of claim 16 where the venom is from a snake, a bee, a spider, a platypus, a scorpion, and ant, a Portuguese man-of-war, or a microorganism.
22. The method of claim 16 wherein the venom is from a venomous snake dangerous to humans.
23. The method of claim 16 wherein the material having protease activity or
carboxypeptidase activity is a protease having at least 40% identity to human
carboxypeptidase of CBPA3_HUMAN.
24. The method of claim 16 wherein the material having protease activity is selected from the group consisting of: carboxypeptidase, chymotrypsin, chymase, tryptase or papain, or sequences at least 80% identical thereto.
25. The method of claim 24 wherein the carboxypeptidase is carboxypeptidase A, or a sequence at least 80% identical thereto.
26. A kit for treating toxicity resulting from envenomation, comprising a preparation of a purified protease in an injectable formulation and an injector device for delivering the formulation intradermally or subcutaneously.