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1. WO2007148106 - DETECTION OF ACETYLATION OF PROKARYOTIC PROTEINS BY MASS SPECTROMETRY

Publication Number WO/2007/148106
Publication Date 27.12.2007
International Application No. PCT/GB2007/002332
International Filing Date 25.06.2007
IPC
G01N 33/68 2006.01
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
68involving proteins, peptides or amino acids
CPC
C07K 14/35
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
195from bacteria
35from Mycobacteriaceae (F)
C12Q 1/48
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
48involving transferase
G01N 2333/91045
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
2333Assays involving biological materials from specific organisms or of a specific nature
90Enzymes; Proenzymes
91Transferases (2.)
91045Acyltransferases (2.3)
G01N 2500/00
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
2500Screening for compounds of potential therapeutic value
G01N 33/6842
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
68involving proteins, peptides or amino acids
6803General methods of protein analysis not limited to specific proteins or families of proteins
6842Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
G01N 33/6851
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
68involving proteins, peptides or amino acids
6803General methods of protein analysis not limited to specific proteins or families of proteins
6848Methods of protein analysis involving mass spectrometry
6851Methods of protein analysis involving laser desorption ionisation mass spectrometry
Applicants
  • THE ROYAL VETERINARY COLLEGE [GB]/[GB] (AllExceptUS)
  • MATTOW, Jens [DE]/[DE] (UsOnly)
  • STOKER, Neil, Graham [GB]/[GB] (UsOnly)
  • JUNGBLUT, Peter, Roman [AT]/[DE] (UsOnly)
  • RISON, Stuart, Christopher, Gorthorn [GB]/[GB] (UsOnly)
Inventors
  • MATTOW, Jens
  • STOKER, Neil, Graham
  • JUNGBLUT, Peter, Roman
  • RISON, Stuart, Christopher, Gorthorn
Agents
  • MILES, John
Priority Data
0612564.524.06.2006GB
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) DETECTION OF ACETYLATION OF PROKARYOTIC PROTEINS BY MASS SPECTROMETRY
(FR) ACÉTYLATION
Abstract
(EN)
A method of identifying an N-terminally acetylated protein in a bacterium, the method comprising providing details of at least one putative translation start site (TSS) for at least one protein expressed in the bacterium, confirming the actual TSS of the at least one protein using mass spectrometry (MS), and determining whether the at least one protein is N-terminally acetylated using MS. A method of identifying an N-terminally acetylated protein in a bacterium, the method comprising providing a mutant strain of the bacterium comprising at least one protein N-acetyl transferase (pNAT) in mutant form, providing a wild-type strain of the bacterium comprising the at least one pNAT in wild-type form, and identifying a protein that is differentially N-terminal acetylated between the mutant and wild-type bacterial strains. A method of identifying a drug discovery target in a pathogenic bacterium, the method comprising determining at least one property of an N-terminally acetylated protein expressed in a pathogenic bacterium and which is relevant to the pathogenicity of the pathogenic bacterium. A method of identifying a bacterial pNAT. A method of screening for an inhibitor of a bacterial pNAT.
(FR)
L'invention concerne un procédé d'identification d'une protéine acétylée en extrémité N dans une bactérie, ledit procédé consistant à obtenir des détails concernant au moins un site de départ de traduction (TSS) présumé pour au moins une protéine exprimée dans la bactérie, confirmer le TSS réel de ladite ou desdites protéines en utilisant la spectrométrie de masse (MS) et déterminer si ladite ou lesdites protéines sont acétylées en extrémité N en utilisant la MS; un procédé d'identification d'une protéine acétylée en extrémité N dans une bactérie, ledit procédé consistant à obtenir une souche mutante de la bactérie comprenant au moins une protéine N-acétyl transférase (pNAT) sous la forme mutante, obtenir une souche de type sauvage de la bactérie comprenant ladite ou lesdites pNAT sous la forme de type sauvage et identifier une protéine qui est acétylée en extrémité N de façon différentielle entre la souche bactérienne mutante et la souche bactérienne de type sauvage; un procédé d'indentification d'une cible de découverte de médicament dans une bactérie pathogène, ledit procédé consistant à déterminer au moins une propriété d'une protéine acétylée en extrémité N exprimée dans une bactérie pathogène et qui est impliquée dans la pathogénicité de la bactérie pathogène; un procédé d'identification d'une pNAT bactérienne; et un procédé de recherche par criblage d'un inhibiteur d'une pNAT bactérienne.
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