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1. WO2007147945 - MODIFIED BETA-LACTAMASE AND METHOD FOR ITS PREPARATION

Publication Number WO/2007/147945
Publication Date 27.12.2007
International Application No. PCT/FI2007/050372
International Filing Date 19.06.2007
IPC
C12N 9/86 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
14Hydrolases (3.)
78acting on carbon to nitrogen bonds other than peptide bonds (3.5)
86acting on amide bonds in cyclic amides, e.g. penicillinase
A61K 38/46 2006.01
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
38Medicinal preparations containing peptides
16Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
43Enzymes; Proenzymes; Derivatives thereof
46Hydrolases (3)
C12N 1/21 2006.01
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
20Bacteria; Culture media therefor
21modified by introduction of foreign genetic material
CPC
A61K 38/00
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
38Medicinal preparations containing peptides
A61K 38/46
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
38Medicinal preparations containing peptides
16Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
43Enzymes; Proenzymes; Derivatives thereof
46Hydrolases (3)
A61P 1/00
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
1Drugs for disorders of the alimentary tract or the digestive system
A61P 39/02
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
39General protective or antinoxious agents
02Antidotes
C07K 14/32
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
195from bacteria
32from Bacillus (G)
C12N 9/86
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes; Proenzymes; Compositions thereof
14Hydrolases (3)
78acting on carbon to nitrogen bonds other than peptide bonds (3.5)
86acting on amide bonds in cyclic amides, e.g. penicillinase ; (3.5.2)
Applicants
  • IPSAT THERAPIES OY [FI]/[FI] (AllExceptUS)
  • KÄÄRIÄINEN, Susanna [FI]/[FI] (UsOnly)
  • WICKSTRAND, Nina [DE]/[FI] (UsOnly)
  • KOSKI, Pertti [FI]/[FI] (UsOnly)
Inventors
  • KÄÄRIÄINEN, Susanna
  • WICKSTRAND, Nina
  • KOSKI, Pertti
Agents
  • KOLSTER OY AB
Priority Data
2006543121.06.2006FI
Publication Language English (EN)
Filing Language English (EN)
Designated States
Title
(EN) MODIFIED BETA-LACTAMASE AND METHOD FOR ITS PREPARATION
(FR) BÊTA-LACTAMASE MODIFIÉE ET SON PROCÉDÉ DE PRÉPARATION
Abstract
(EN)
The invention relates to targeted post translational modification of metallo-beta-lactamase by truncation and insertion of a dipeptide at the amino terminal end to reduce amino terminal heterogeneity in a recombinant DNA production system. A protein K-T-E-ΔBL is expressed, and modified by host proteases to E-ΔBL. Appropriate nucleotide molecules, vectors and hosts are also described. E-ΔBL is useful in a pharmaceutical composition for treating antibiotic induced adverse effects in the intestine of patients treated with beta-lactam antibiotics.
(FR)
La présente invention concerne la modification ciblée après traduction de la métallo-bêta-lactamase par troncature et insertion d'un dipeptide au niveau de l'extrémité N-terminale afin de réduire l'hétérogénéité du N-terminal dans un système de production d'ADN recombinant. Selon l'invention, une protéine K-T-E-ΔBL est exprimée et modifiée en E-ΔBL par les protéases de l'hôte. L'invention concerne également des molécules nucléotidiques, des vecteurs et des hôtes appropriés. E-ΔBL est utilisable dans une composition pharmaceutique destinée au traitement des effets secondaires défavorables des antibiotiques dans l'intestin de patients traités par des antibiotiques bêta-lactames.
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