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1. WO2007144393 - MASHING PROCESS

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[ EN ]

CLAIMS
1. A process for producing a brewers wort comprising forming a mash from a grist, and contacting said mash with a pullulanase, wherein said pullulanase has an amino acid sequence which
a) is at least 50% identical to the amino acid sequence shown in SEQ ID NO:3; or b) is encoded by a nucleic acid sequence which hybridizes under low stringency conditions with
i) a complementary strand of a nucleic acid sequence encoding the amino acid sequence shown in SEQ ID NO:3; or
ii) a subsequence of (i) of at least 100 nucleotides.

2. The process according to any of the preceding claims wherein the pullulanase is derived from Bacillus acidopullulyticus.

3. The process according to any of the preceding claims further comprising contacting said mash with a glucoamylase and/or alpha-amylase.

4. The process according to any of the preceding claims wherein the glucoamylase and/or alpha-amylase is derived from Aspergillus niger or Talaromyces emersonii.

5. The process according to any of the preceding claims further comprising contacting said mash with an enzyme selected from the group consisting of cellulase, isoamylase, xylanase and protease.

6. The process according to any of the preceding claims, wherein the grist comprises malted and/or unmalted grain.

7. The process according to any of the preceding claims, wherein the unmalted grain and/or the malted grain is selected from the list consisting of barley, wheat, rye, sorghum, millet, corn and rice.

8. The process according to any of the preceding claims, wherein the malted grain comprises malted grain selected from malted barley, wheat, rye, sorghum, millet, corn, and rice.

9. The process according to any of the preceding claims, wherein the wort is concentrated and/or dried.

10. The process according to any of the preceding claims, further comprising fermenting the wort to obtain an alcoholic beverage.

1 1. The process according the preceding claim wherein the alcoholic beverage is a beer.

12. The process according to any of the preceding claims, wherein the beer is ale, strong ale, bitter, stout, porter, lager, export beer, malt liquor, barley wine, happoushu, high-alcohol beer, low-alcohol beer, low-calorie beer or light beer.

13. A wort produced by the process according to any of the preceding claims.

14. A concentrated and/or dried wort according to any of the preceding claims.

15. A beer produced from the wort according to any of the preceding claims.

16. A composition suitable for use in the process according to any of the preceding claims, said composition comprising a pullulanase. a glucoamylase and optionally an alpha-amylase, wherein said pullulanase has an amino acid sequence which
a) is at least 50% identical to the amino acid sequence shown in SEQ ID NO:3. or b) is encoded by a nucleic acid sequence which hybridizes under low stringency conditions with
i) a complementary strand of a nucleic acid sequence encoding the amino acid sequence shown in SEQ ID NO:3. or
ii) a subsequence of (i) of at least 100 nucleotides.

17. The composition according to the preceding claim wherein the glucoamylase and/or the alpha-amylase is derived from Aspergillus niger or Talaromyces emersonii.